Nicotinic and muscarinic acetylcholine responses in the embryo chick ciliary ganglion cells

Katsutoshi Furukawa, Yumiko Abe, Masaru Sorimachi, Norio Akaike

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nicotinic and muscarinic acetylcholine (ACh) responses were investigated in acutely dissociated chick ciliary ganglion neurons using the nystatin perforated patch clamp technique. ACh-induced a rapid transient inward current in 100% of the neurons at a holding potential of -60 mV. This rapid inward current was mimicked by nicotine but not by muscarine. The reversal potential of the rapid inward current was + 10.5 mV and the current was inhibited by d-tubocurarine and hexamethonium in a dose-dependent manner. In 57.6% of neurons, a slow inward current was also induced by ACh at a holding potential of -20 mV. This slow inward current was mimicked by muscarine but not by nicotine. The slow inward current became smaller at a hyperpolarized potential but not reversed, being consistent with the fact that this current was elicited by the inhibition of M-current. p-Fluorohexa-hydrosiladifenidol (P-F-HHSiD) strongly inhibited the slow inward current, suggesting that the current was elicited by the activation of M3 receptors.

Original languageEnglish
Pages (from-to)185-190
Number of pages6
JournalBrain research
Volume657
Issue number1-2
DOIs
Publication statusPublished - 1994 Sep 19

Keywords

  • Acetylcholine response
  • Chick embryo
  • Ciliary ganglion
  • Muscarinic M receptor

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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