Nicotinic acid adenine dinucleotide phosphate-induced Ca2+ release. Interactions among distinct Ca2+ mobilizing mechanisms in starfish oocytes

Luigia Santella, Keiichiro Kyozuka, Armando A. Genazzani, Laura De Riso, Ernesto Carafoli

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

An intracellular mechanism activated by nicotinic acid adenine dinucleotide phosphate (NAADP+) contributes to intracellular Ca2+ release alongside inositol 1,4,5-trisphosphate (Ins-P3) and ryanodine receptors. The NAADP+-sensitive mechanism has been shown to be operative in sea urchin eggs, ascidian eggs, and pancreatic acinar cells. Furthermore, most mammalian cell types can synthesize NAADP+, with nicotinic acid and NADP+ as precursors. In this contribution, NAADP+ induced Ca2+ release has been investigated in starfish oocytes. Uncaging of injected NAADP+ induced Ca2+ mobilization in both immature oocytes and in oocytes matured by the hormone 1-methyladenine (1-MA). The role of extracellular Ca2+ in NAADP+-induced Ca2+ mobilization, which was minor in immature oocytes, was instead essential in mature oocytes. Thus, the NAADP+sensitive Ca2+ pool, which is known to be distinct from those sensitive to inositol 1,4,5-trisphosphate or cyclic ADPribose, apparently migrated closer to (or became part of) the plasma membrane during the maturation process. Inhibition of both Ins-P3 and ryanodine receptors, but not of either alone, substantially inhibited NAADP+-induced Ca2+ mobilization in both immature and mature oocytes. The data also suggest that NAADP+-induced Ca2+ mobilization acted as a trigger for Ca2+ release via Ins-P3 and ryanodine receptors.

Original languageEnglish
Pages (from-to)8301-8306
Number of pages6
JournalJournal of Biological Chemistry
Volume275
Issue number12
DOIs
Publication statusPublished - 2000 Mar 24

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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