Nicotine reduced MMP-9 expression in the primary porcine tenocytes exposed to cyclic stretch

Taku Hatta, Hirotaka Sano, Naoya Sakamoto, Koshi N. Kishimoto, Masaaki Sato, Eiji Itoi

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15 Citations (Scopus)


Nicotine is one of the major chemical components of the cigarette smoke, which has been known as a risk factor for tendon ruptures including rotator cuff tears. This study investigated the effect of nicotine on tenocytes under cyclic-stretched condition. Particularly, we focused on the morphologic changes of tenocytes and their expression of MMPs. Primary porcine tenocytes were obtained from the infraspinatus tendon. The cells were cultured on elastic chambers under static or cyclic-stretched condition for 24 h in the existence of nicotine (0, 1, 10, and 100 μM). Cell shape, gene expression of collagen type I and III, MMPs (-1, -2, -3, -9, and -13) and TIMPs (-1, -2, and -3) and enzyme activity of MMP-9 were analyzed using immunohistochemistry, RT-PCR, and zymography. Tenocytes exposed to nicotine represented significantly decreased gene expressions in MMP-9 (p < 0.001) and TIMP-3 (p < 0.05) under the cyclic stretch. Enzymatic activity of MMP-9 was also reduced by nicotine exposure in a dose-dependent manner (p < 0.001). The down-regulation of MMP and TIMP expression by nicotine shown in our in vitro experiment might deteriorate normal metabolism of the tendon. These mechanisms might affect the mechanical properties of the extracellular matrix of the rotator cuff tendon.

Original languageEnglish
Pages (from-to)645-650
Number of pages6
JournalJournal of Orthopaedic Research
Issue number4
Publication statusPublished - 2013 Apr


  • matrix metalloproteinase
  • nicotine
  • tendinopathy
  • tenocyte

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine


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