Nicotine overrides DNA damage-induced G1/S restriction in lung cells

Takashi Nishioka, Daisuke Yamamoto, Tongbo Zhu, Jinjin Guo, Sung Hoon Kim, Chang Yan Chen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

As an addictive substance, nicotine has been suggested to facilitate pro-survival activities (such as anchorage-independent growth or angiogenesis) and the establishment of drug resistance to anticancer therapy. Tobacco smoking consists of a variety of carcinogens [such as benzopyrene (BP) and nitrosamine derivatives] that are able to cause DNA double strand breaks. However, the effect of nicotine on DNA damage-induced checkpoint response induced by genotoxins remains unknown. In this study, we investigated the events occurred during G1 arrest induced by γ-radiation or BP in nicotine-treated murine or human lung epithelial cells. DNA synthesis was rapidly inhibited after exposure to γ-radiation or BP treatment, accompanied with the activation of DNA damage checkpoint. When these cells were co-treated with nicotine, the growth restriction was compromised, manifested by upregulation of cyclin D and A, and attenuation of Chk2 phosphorylation. Knockdown of cyclin D or Chk2 by the siRNAs blocked nicotine-mediated effect on DNA damage checkpoint activation. However, nicotine treatment appeared to play no role in nocodazole-induced mitotic checkpoint activation. Overall, our study presented a novel observation, in which nicotine is able to override DNA damage checkpoint activated by tobacco-related carcinogen BP or γ-irradiation. The results not only indicates the potentially important role of nicotine in facilitating the establishment of genetic instability to promote lung tumorigenesis, but also warrants a dismal prognosis for cancer patients who are smokers, heavily exposed second-hand smokers or nicotine users.

Original languageEnglish
Article numbere18619
JournalPloS one
Volume6
Issue number4
DOIs
Publication statusPublished - 2011 May 12
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

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    Nishioka, T., Yamamoto, D., Zhu, T., Guo, J., Kim, S. H., & Chen, C. Y. (2011). Nicotine overrides DNA damage-induced G1/S restriction in lung cells. PloS one, 6(4), [e18619]. https://doi.org/10.1371/journal.pone.0018619