Nicotinamide is a specific inhibitor of dark-operative protochlorophyllide oxidoreductase, a nitrogenase-like enzyme, from Rhodobacter capsulatus

Jiro Nomata, Toru Kondo, Shigeru Itoh, Yuichi Fujita

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Dark-operative protochlorophyllide oxidoreductase (DPOR) is a nitrogenase-like enzyme consisting of two components, L-protein as a reductase component and NB-protein as a catalytic component. Elucidation of the crystal structures of NB-protein (Muraki et al., Nature 2010, 465: 110-114) has enabled us to study its reaction mechanism in combination with biochemical analysis. Here we demonstrate that nicotinamide (NA) inhibits DPOR activity by blocking the electron transfer from L-protein to NB-protein. A reaction scheme of DPOR, in which the binding of protochlorophyllide (Pchlide) to the NB-protein precedes the electron transfer from the L-protein, is proposed based on the NA effects.

Original languageEnglish
Pages (from-to)3142-3147
Number of pages6
JournalFEBS Letters
Volume587
Issue number18
DOIs
Publication statusPublished - 2013 Sep 17
Externally publishedYes

Keywords

  • Bacteriochlorophyll
  • Chlorophyll
  • FeS cluster
  • Nitrogenase
  • Protochlorophyllide

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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