Nicotinamide inhibits inducible nitric oxide synthase mRNA primary rat glial cells

Miki Fujimura, Teiji Tominaga, Takashi Yoshimoto

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Nitric oxide (NO) exerts cytotoxic effects on various cells including neuronal cells. Glial NO production, mediated via induction of inducible NO synthase (iNOS), enhances neurotoxicity associated with the N-methyl-D- aspartate (NMDA) receptor. The present study examined whether nicotinamide, an inhibitor of poly (ADP-ribose) synthetase, inhibits NO formation in primary culture of rat glial cells. Nicotinamide (5-20 mM) suppressed iNOS mRNA expression and subsequent NO formation, which were induced by the combination of interferon-γ and lipopolysaccharide, in a dose dependent manner. In addition, high-concentration (20 mM) nicotinamide decreased mRNA of interferon regulatory factor-1, a transcription factor which plays a major role in iNOS mRNA induction. These results suggest thai nicotinamide may have protective effect on glial NO-related pathologies by preventing iNOS mRNA induction.

Original languageEnglish
Pages (from-to)107-110
Number of pages4
JournalNeuroscience Letters
Volume228
Issue number2
DOIs
Publication statusPublished - 1997 Jun 6

Keywords

  • Glial cells
  • Inducible nitric oxide synthase
  • Interferon-γ
  • Lipopolysaccharide
  • Nicotinamide
  • Nitric oxide
  • Nitric oxide synthase
  • Poly (ADP- ribose) synthetase

ASJC Scopus subject areas

  • Neuroscience(all)

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