Abstract
Nitric oxide (NO) exerts cytotoxic effects on various cells including neuronal cells. Glial NO production, mediated via induction of inducible NO synthase (iNOS), enhances neurotoxicity associated with the N-methyl-D- aspartate (NMDA) receptor. The present study examined whether nicotinamide, an inhibitor of poly (ADP-ribose) synthetase, inhibits NO formation in primary culture of rat glial cells. Nicotinamide (5-20 mM) suppressed iNOS mRNA expression and subsequent NO formation, which were induced by the combination of interferon-γ and lipopolysaccharide, in a dose dependent manner. In addition, high-concentration (20 mM) nicotinamide decreased mRNA of interferon regulatory factor-1, a transcription factor which plays a major role in iNOS mRNA induction. These results suggest thai nicotinamide may have protective effect on glial NO-related pathologies by preventing iNOS mRNA induction.
| Original language | English |
|---|---|
| Pages (from-to) | 107-110 |
| Number of pages | 4 |
| Journal | Neuroscience Letters |
| Volume | 228 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 1997 Jun 6 |
Keywords
- Glial cells
- Inducible nitric oxide synthase
- Interferon-γ
- Lipopolysaccharide
- Nicotinamide
- Nitric oxide
- Nitric oxide synthase
- Poly (ADP- ribose) synthetase
ASJC Scopus subject areas
- Neuroscience(all)