Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes

Atsushi Nishida, Hiroshi Iwata, Yukitsuka Kudo, Tsutomu Kobayashi, Yuzo Matsuoka, Yoshikatsu Kanai, Hitoshi Endou

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    To elucidate the mechanisms of neuroprotective action of nicergoline, we examined its effect on glutamate transport in rat cortical synaptosomes and cloned glutamate transporters. In synaptosomes, nicergoline enhanced the glutamate uptake at 1-10 μM in standard medium and suppressed the increase of extracellular glutamate by reversed transport in low Na+ medium. Apparent increase of extracellular glutamate concentration by dihydrokinate, an inhibitor of glial glutamate transporter GLT-1, was antagonized by nicergoline. In Xenopus oocytes expressing mouse neuronal glutamate transporter (mEAAC1), the glutamate-induced inward current was enhanced by nicergoline. These results suggest that nicergoline reduces the extracellular glutamate concentration through its effect on glutamate transporters.

    Original languageEnglish
    Pages (from-to)817-820
    Number of pages4
    JournalBiological and Pharmaceutical Bulletin
    Volume27
    Issue number6
    DOIs
    Publication statusPublished - 2004 Jun

    Keywords

    • Glutamate reversed transport
    • Glutamate transporter
    • Glutamate uptake
    • Nicergoline
    • Synaptosome
    • Xenopus oocyte

    ASJC Scopus subject areas

    • Pharmacology
    • Pharmaceutical Science

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