Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes

Atsushi Nishida; Hiroshi Iwata; Yukitsuka Kudo; Tsutomu Kobayashi; Yuzo Matsuoka; Yoshikatsu Kanai; Hitoshi Endou

doi:10.1248/bpb.27.817

Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes

Atsushi Nishida, Hiroshi Iwata, Yukitsuka Kudo, Tsutomu Kobayashi, Yuzo Matsuoka, Yoshikatsu Kanai, Hitoshi Endou

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

Abstract

To elucidate the mechanisms of neuroprotective action of nicergoline, we examined its effect on glutamate transport in rat cortical synaptosomes and cloned glutamate transporters. In synaptosomes, nicergoline enhanced the glutamate uptake at 1-10 μM in standard medium and suppressed the increase of extracellular glutamate by reversed transport in low Na⁺ medium. Apparent increase of extracellular glutamate concentration by dihydrokinate, an inhibitor of glial glutamate transporter GLT-1, was antagonized by nicergoline. In Xenopus oocytes expressing mouse neuronal glutamate transporter (mEAAC1), the glutamate-induced inward current was enhanced by nicergoline. These results suggest that nicergoline reduces the extracellular glutamate concentration through its effect on glutamate transporters.

Original language	English
Pages (from-to)	817-820
Number of pages	4
Journal	Biological and Pharmaceutical Bulletin
Volume	27
Issue number	6
DOIs	https://doi.org/10.1248/bpb.27.817
Publication status	Published - 2004 Jun

Keywords

Glutamate reversed transport
Glutamate transporter
Glutamate uptake
Nicergoline
Synaptosome
Xenopus oocyte

ASJC Scopus subject areas

Pharmacology
Pharmaceutical Science

Access to Document

10.1248/bpb.27.817

Fingerprint Dive into the research topics of 'Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes'. Together they form a unique fingerprint.

Cite this

@article{c4b11b62049846bfb7a34d0ef8faa879,

title = "Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes",

abstract = "To elucidate the mechanisms of neuroprotective action of nicergoline, we examined its effect on glutamate transport in rat cortical synaptosomes and cloned glutamate transporters. In synaptosomes, nicergoline enhanced the glutamate uptake at 1-10 μM in standard medium and suppressed the increase of extracellular glutamate by reversed transport in low Na+ medium. Apparent increase of extracellular glutamate concentration by dihydrokinate, an inhibitor of glial glutamate transporter GLT-1, was antagonized by nicergoline. In Xenopus oocytes expressing mouse neuronal glutamate transporter (mEAAC1), the glutamate-induced inward current was enhanced by nicergoline. These results suggest that nicergoline reduces the extracellular glutamate concentration through its effect on glutamate transporters.",

keywords = "Glutamate reversed transport, Glutamate transporter, Glutamate uptake, Nicergoline, Synaptosome, Xenopus oocyte",

author = "Atsushi Nishida and Hiroshi Iwata and Yukitsuka Kudo and Tsutomu Kobayashi and Yuzo Matsuoka and Yoshikatsu Kanai and Hitoshi Endou",

year = "2004",

month = jun,

doi = "10.1248/bpb.27.817",

language = "English",

volume = "27",

pages = "817--820",

journal = "Biological and Pharmaceutical Bulletin",

issn = "0918-6158",

publisher = "Pharmaceutical Society of Japan",

number = "6",

}

TY - JOUR

T1 - Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes

AU - Nishida, Atsushi

AU - Iwata, Hiroshi

AU - Kudo, Yukitsuka

AU - Kobayashi, Tsutomu

AU - Matsuoka, Yuzo

AU - Kanai, Yoshikatsu

AU - Endou, Hitoshi

PY - 2004/6

Y1 - 2004/6

N2 - To elucidate the mechanisms of neuroprotective action of nicergoline, we examined its effect on glutamate transport in rat cortical synaptosomes and cloned glutamate transporters. In synaptosomes, nicergoline enhanced the glutamate uptake at 1-10 μM in standard medium and suppressed the increase of extracellular glutamate by reversed transport in low Na+ medium. Apparent increase of extracellular glutamate concentration by dihydrokinate, an inhibitor of glial glutamate transporter GLT-1, was antagonized by nicergoline. In Xenopus oocytes expressing mouse neuronal glutamate transporter (mEAAC1), the glutamate-induced inward current was enhanced by nicergoline. These results suggest that nicergoline reduces the extracellular glutamate concentration through its effect on glutamate transporters.

AB - To elucidate the mechanisms of neuroprotective action of nicergoline, we examined its effect on glutamate transport in rat cortical synaptosomes and cloned glutamate transporters. In synaptosomes, nicergoline enhanced the glutamate uptake at 1-10 μM in standard medium and suppressed the increase of extracellular glutamate by reversed transport in low Na+ medium. Apparent increase of extracellular glutamate concentration by dihydrokinate, an inhibitor of glial glutamate transporter GLT-1, was antagonized by nicergoline. In Xenopus oocytes expressing mouse neuronal glutamate transporter (mEAAC1), the glutamate-induced inward current was enhanced by nicergoline. These results suggest that nicergoline reduces the extracellular glutamate concentration through its effect on glutamate transporters.

KW - Glutamate reversed transport

KW - Glutamate transporter

KW - Glutamate uptake

KW - Nicergoline

KW - Synaptosome

KW - Xenopus oocyte

UR - http://www.scopus.com/inward/record.url?scp=16644394840&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=16644394840&partnerID=8YFLogxK

U2 - 10.1248/bpb.27.817

DO - 10.1248/bpb.27.817

M3 - Article

C2 - 15187425

AN - SCOPUS:16644394840

VL - 27

SP - 817

EP - 820

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 6

ER -

Nicergoline enhances glutamate uptake via glutamate transporters in rat cortical synaptosomes

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this