NF-κB-dependent induction of osteoprotegerin by Porphyromonas gingivalis in endothelial cells

Michiyo Kobayashi-Sakamoto, Kimiharu Hirose, Emiko Isogai, Itsuo Chiba

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Porphyromonas gingivalis is a major etiological pathogen of adult periodontitis characterized by alveolar bone resorption. Vascular endothelial cells supply many inflammatory cytokines into periodontal tissue. However, whether the cells contribute to bone metabolism in periodontitis remains unknown. In this study, we investigated the effect of P. gingivalis on osteoprotegerin (OPG) and receptor activator of NF-κB ligand (RANKL) production, both of which are key regulators of bone metabolism, in human microvascular endothelial cells (HMVECs). We showed that P. gingivalis upregulated expression of OPG but not RANKL mRNA in HMVEC. P. gingivalis induced NF-κB activation, and the induction of OPG in HMVEC by the pathogen was blocked by the inhibitors of NF-κB. In addition, incubation of OPG with P. gingivalis supernatant resulted in loss of the protein. These results indicate that P. gingivalis-stimulated HMVEC secrete OPG via a NF-κB-dependent pathway, while the OPG is partly degraded by the bacteria. Thus, microvascular endothelial cells can act as a source of OPG and thereby may play an important role in regulating bone metabolism in periodontitis.

Original languageEnglish
Pages (from-to)107-112
Number of pages6
JournalBiochemical and biophysical research communications
Volume315
Issue number1
DOIs
Publication statusPublished - 2004 Feb 27

Keywords

  • Bone resorption
  • Endothelial cells
  • Inflammatory cytokines
  • Lipopolysaccharide
  • NF-κB
  • Osteoprotegerin
  • Periodontitis
  • Porphyromonas gingivalis
  • Protease
  • Receptor activator of NF-κB ligand

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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