New polymorphic microsatellite markers in the human MHC class I region

G. Tamiya, T. Shiina, A. Oka, M. Tomizawa, M. Ota, Y. Katsuyama, M. Yoshitome, S. Makino, M. Kimura, Hidetoshi Inoko

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The human major histocompatibility complex (MHC) class I region is believed to contain a large number of genes encoding susceptible factors for diseases such as Behcet's disease, Graves disease and psoriasis vulgaris. To identify the causative genes of those diseases, we have conducted large-scale genomic sequencing and determined the 1.8 Mb entire HLA class I region from the MICB gene to the HLA-F gene. During the course of genomic sequencing, a total of 731 microsatellite sequences with dinucleotide to pentanucleotide repeats were found in this region. Previously, we reported that 26 microsatellites between MICB and S on the most centromeric side of the class I region, and between HSR1 and HLA-92/L in the midst of the class I region were highly polymorphic, and served as excellent genetic markers. In this paper, in order to fill the gaps with no known polymorphic microsatellites available in the HLA class I region, 12 new polymorphic microsatellite markers were recruited from the 1.8 Mb region including the remaining class I segments, namely between S and HSR1, and between HLA-92/L and HLA-F. The average number of alleles at these new microsatellite loci was 8.2 with a polymorphism content value (PIC) of 0.63. These 38 markers in total almost uniformly interspersed in the HLA class I region will enable us to search precisely for the location of disease susceptible loci within the HLA class I region by association and for linkage analyses.

Original languageEnglish
Pages (from-to)221-228
Number of pages8
JournalTissue Antigens
Volume54
Issue number3
DOIs
Publication statusPublished - 1999

Keywords

  • 6p21.3
  • HLA region
  • Human MHC class I
  • Japanese population
  • Microsatellite repeat
  • Polymorphic marker

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Biochemistry
  • Genetics

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  • Cite this

    Tamiya, G., Shiina, T., Oka, A., Tomizawa, M., Ota, M., Katsuyama, Y., Yoshitome, M., Makino, S., Kimura, M., & Inoko, H. (1999). New polymorphic microsatellite markers in the human MHC class I region. Tissue Antigens, 54(3), 221-228. https://doi.org/10.1034/j.1399-0039.1999.540302.x