New PCR-RFLP analysis for the mouse Tnfsf6gld gene caused by a point mutation in the Tnfsf6 [tumor necrosis factor (ligand) superfamily, member 6] locus

Norio Masui, Yumie Takagi, Tetsu Nishikawa, Makoto Yanabe, Masato Nose, Katsunori Sato

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation.

Original languageEnglish
Pages (from-to)501-503
Number of pages3
JournalExperimental Animals
Volume51
Issue number5
DOIs
Publication statusPublished - 2002 Oct

Keywords

  • Modified primer
  • PCR-RFLP
  • Tnfsf6 mutation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

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