Abstract
A new polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was developed for genetic typing of the mouse Tnfsf6gld mutation. An artificial restriction site was introduced to the mouse Tnfsf6gld mutation by PCR amplification using a modified primer. The three genotypes of the Tnfsf6 locus (Tnfsf6gld/Tnfsf6gld, Tnfsf6gld/+, and +Tnfsf6-gld/+Tnfsf6-gld) could be distinguished clearly and easily. This PCR-RFLP analysis was found to be useful for the identification of the mouse Tnfsf6gld mutation.
Original language | English |
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Pages (from-to) | 501-503 |
Number of pages | 3 |
Journal | Experimental Animals |
Volume | 51 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2002 Oct |
Keywords
- Modified primer
- PCR-RFLP
- Tnfsf6 mutation
ASJC Scopus subject areas
- Animal Science and Zoology
- Biochemistry, Genetics and Molecular Biology(all)
- veterinary(all)