New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat

Sufan Wu; Yoshihisa Suzuki; Masaaki Kitada; Kazuya Kataoka; Miyako Kitaura; Hirotomi Chou; Yoshihiko Nishimura; Chizuka Ide

doi:10.1016/S0304-3940(01)02488-0

New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat

Sufan Wu, Yoshihisa Suzuki, Masaaki Kitada, Kazuya Kataoka, Miyako Kitaura, Hirotomi Chou, Yoshihiko Nishimura, Chizuka Ide

MED - Medical Sciences

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

Here we report a novel method of supplying cultured neurosphere cells to the injured spinal cord, by injection of cells into the cerebrospinal fluid (CSF) through the fourth ventricle or cisterna magna. Hippocampus-derived neurosphere cells, isolated from a transgenic rat fetus expressing green fluorescent protein, were transplanted into the CSF of a rat with spinal cord injury. It was found that injected cells were extensively transported by CSF within the subarachnoidal space, and survived as clusters on the pial surface of the spinal cord. The most notable finding was that a large number of injected cells migrated into the lesion site and integrated into the injured spinal cord tissues.

Original language	English
Pages (from-to)	81-84
Number of pages	4
Journal	Neuroscience Letters
Volume	318
Issue number	2
DOIs	https://doi.org/10.1016/S0304-3940(01)02488-0
Publication status	Published - 2002 Jan 25

Keywords

Cerebrospinal fluid
Green fluorescent protein
Hippocampus
Neurosphere
Transgenic

ASJC Scopus subject areas

Neuroscience(all)

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New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat. / Wu, Sufan; Suzuki, Yoshihisa; Kitada, Masaaki; Kataoka, Kazuya; Kitaura, Miyako; Chou, Hirotomi; Nishimura, Yoshihiko; Ide, Chizuka.

In: Neuroscience Letters, Vol. 318, No. 2, 25.01.2002, p. 81-84.

Research output: Contribution to journal › Article › peer-review

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