TY - JOUR
T1 - New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat
AU - Wu, Sufan
AU - Suzuki, Yoshihisa
AU - Kitada, Masaaki
AU - Kataoka, Kazuya
AU - Kitaura, Miyako
AU - Chou, Hirotomi
AU - Nishimura, Yoshihiko
AU - Ide, Chizuka
N1 - Funding Information:
This is contribution No.IS-2301 from GIGCAS. This work was financially supported by the National Natural Science Foundation of China (Grant No. 41572032), Natural Science Foundation for Distinguished Young Scientists of Guangdong Province (Grant No. 2016A030306034), Natural Science Foundation of Guangdong Province, China (Grant No. S2013030014241), Special Project on the Integration of Industry, Education and Research of Guangdong Province (Grant No. 2013B091500077), Youth Innovation Promotion Association CAS (Grant No. 2014324), and CAS/SAFEA International Partnership Program for Creative Research Teams (Grant No. 20140491534).
PY - 2002/1/25
Y1 - 2002/1/25
N2 - Here we report a novel method of supplying cultured neurosphere cells to the injured spinal cord, by injection of cells into the cerebrospinal fluid (CSF) through the fourth ventricle or cisterna magna. Hippocampus-derived neurosphere cells, isolated from a transgenic rat fetus expressing green fluorescent protein, were transplanted into the CSF of a rat with spinal cord injury. It was found that injected cells were extensively transported by CSF within the subarachnoidal space, and survived as clusters on the pial surface of the spinal cord. The most notable finding was that a large number of injected cells migrated into the lesion site and integrated into the injured spinal cord tissues.
AB - Here we report a novel method of supplying cultured neurosphere cells to the injured spinal cord, by injection of cells into the cerebrospinal fluid (CSF) through the fourth ventricle or cisterna magna. Hippocampus-derived neurosphere cells, isolated from a transgenic rat fetus expressing green fluorescent protein, were transplanted into the CSF of a rat with spinal cord injury. It was found that injected cells were extensively transported by CSF within the subarachnoidal space, and survived as clusters on the pial surface of the spinal cord. The most notable finding was that a large number of injected cells migrated into the lesion site and integrated into the injured spinal cord tissues.
KW - Cerebrospinal fluid
KW - Green fluorescent protein
KW - Hippocampus
KW - Neurosphere
KW - Transgenic
UR - http://www.scopus.com/inward/record.url?scp=0037169223&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037169223&partnerID=8YFLogxK
U2 - 10.1016/S0304-3940(01)02488-0
DO - 10.1016/S0304-3940(01)02488-0
M3 - Article
C2 - 11796191
AN - SCOPUS:0037169223
VL - 318
SP - 81
EP - 84
JO - Neuroscience Letters
JF - Neuroscience Letters
SN - 0304-3940
IS - 2
ER -