New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat

Sufan Wu, Yoshihisa Suzuki, Masaaki Kitada, Kazuya Kataoka, Miyako Kitaura, Hirotomi Chou, Yoshihiko Nishimura, Chizuka Ide

Research output: Contribution to journalArticlepeer-review

58 Citations (Scopus)

Abstract

Here we report a novel method of supplying cultured neurosphere cells to the injured spinal cord, by injection of cells into the cerebrospinal fluid (CSF) through the fourth ventricle or cisterna magna. Hippocampus-derived neurosphere cells, isolated from a transgenic rat fetus expressing green fluorescent protein, were transplanted into the CSF of a rat with spinal cord injury. It was found that injected cells were extensively transported by CSF within the subarachnoidal space, and survived as clusters on the pial surface of the spinal cord. The most notable finding was that a large number of injected cells migrated into the lesion site and integrated into the injured spinal cord tissues.

Original languageEnglish
Pages (from-to)81-84
Number of pages4
JournalNeuroscience Letters
Volume318
Issue number2
DOIs
Publication statusPublished - 2002 Jan 25

Keywords

  • Cerebrospinal fluid
  • Green fluorescent protein
  • Hippocampus
  • Neurosphere
  • Transgenic

ASJC Scopus subject areas

  • Neuroscience(all)

Fingerprint Dive into the research topics of 'New method for transplantation of neurosphere cells into injured spinal cord through cerebrospinal fluid in rat'. Together they form a unique fingerprint.

Cite this