New epidermal keratin genes from Xenopus laevis: Hormonal and regional regulation of their expression during anuran skin metamorphosis

Yusuke Watanabe, Hisao Kobayashi, Ken ichi Suzuki, Kaori Kotani, Katsutoshi Yoshizato

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Xenopus larval keratin (XLK) was isolated by gel electrophoresis of proteins of tadpole skin. Screening of an expression cDNA library of tail tissues by specific polyclonal antibodies against XLK produced XLK cDNA (xlk). Its complete nucleotide and predicted amino acid sequences revealed that XLK was a new member of type II keratin. Screening of a cDNA library of adult Xenopus skin using an oligonucleotide probe which had been designed from well-conserved N-terminal amino acid sequences of the rod domain of type I keratin produced two cDNAs, xak-a and xak-b, which were found to be new members of type I keratin gene. Northern blot analysis showed that xlk was expressed exclusively in the larval skin whereas xak-a and xak-b were expressed exclusively in the adult skin. Their expression level was regulated in a region- and metamorphic stage-dependent manner during larval skin development. mRNA in situ hybridization experiments identified the cells that expressed xlk, and xak-a and xak-b as larva-specific epidermal cells (skein cells and basal cells), and adult suprabasal epidermal cells, respectively. These three genes were found to be late responsive to thyroid hormone. Phylogenetic relationships of these keratins with known ones are discussed.

Original languageEnglish
Pages (from-to)339-350
Number of pages12
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1517
Issue number3
DOIs
Publication statusPublished - 2001 Feb 16

Keywords

  • (Amphibian)
  • (Epidermis)
  • (Tadpole)
  • Gene expression
  • Thyroid hormone
  • cDNA

ASJC Scopus subject areas

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

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