New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1',2'-dihydro-γ-carotene, 1',2'-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains

Shinichi Takaichi, Zheng Yu Wang, Mitsuo Umetsu, Tsunenori Nozawa, Keizo Shimada, Michael T. Madigan

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

The complete carotenoid composition of the thermophilic green sulfur bacterium Chlorobium tepidum strain TNO was determined by spectroscopic methods. Major carotenoids were four kinds of carotenes: γ-carotene, chlorobactene, and their 1',2'-dihydro derivatives (1',2'-dihydro-γ-carotene and 1',2'-dihydrochlorobactene). In lesser amounts, hydroxyl γ-carotene, hydroxyl chlorobactene, and their glucoside fatty acid esters were found. The only esterified fatty acid present was laurate, and OH-chlorobactene glucoside laurate is a novel carotenoid. In other strains of C. tepidum, the same carotenoids were found, but the composition varied from strain to strain. The overall pigment composition in cells of strain TNO was 4 mol carotenoids and 40 mol bacteriochlorophyll c per mol bacteriochlorophyll a. The effects of nicotine on carotenoid biosynthesis in C. tepidum differed from those inthe thermophilic green nonsulfur bacterium Chloroflexus aurantiacus.

Original languageEnglish
Pages (from-to)270-276
Number of pages7
JournalArchives of Microbiology
Volume168
Issue number4
DOIs
Publication statusPublished - 1997 Oct 13

Keywords

  • 1',2'-Dihydro-γ-carotene
  • 1',2'-Dihydrochlorobactene
  • Bacteriochlorophyll c
  • Carotenoid biosynthesis
  • Chlorobium tepidum
  • Green sulfur bacteria
  • Nicotine
  • OH-Chlorobactene glucoside ester
  • Thermophily

ASJC Scopus subject areas

  • Microbiology
  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'New carotenoids from the thermophilic green sulfur bacterium Chlorobium tepidum: 1',2'-dihydro-γ-carotene, 1',2'-dihydrochlorobactene, and OH-chlorobactene glucoside ester, and the carotenoid composition of different strains'. Together they form a unique fingerprint.

Cite this