New approaches for the study of orexin function

A. Yamanaka, T. Tsunematsu

Research output: Contribution to journalReview articlepeer-review

9 Citations (Scopus)

Abstract

Orexin is a neuropeptide produced by a specific subset of neurones located in the lateral hypothalamic area. Mice lacking either prepro-orexin or orexin receptor 2, as well as those in which orexin-producing neurones (orexin neurones) are deleted, share a common phenotype: altered sleep-wake regulation and the sudden onset of muscle atonia. These symptoms are similar to the human sleep disorder narcolepsy. In this review, we describe recent advances in the study of orexin function with a particular emphasis on microscopic techniques that better characterise the neuronal networks involving orexin neurones, as well as recent optogenetic approaches that allow for the activation or inhibition of specific neurones by expressing different light-activated proteins. In particular, the use of orexin/halorhodopsin and orexin/channelrhodopsin-2 transgenic mice has demonstrated an important role for orexin neurones in regulating the sleep-wake cycle and state of arousal in vivo. Further refinement of these in vitro and in vivo techniques will allow for a more detailed understanding of the interaction of orexin with other neurotransmitter pathways in the brain.

Original languageEnglish
Pages (from-to)818-824
Number of pages7
JournalJournal of Neuroendocrinology
Volume22
Issue number7
DOIs
Publication statusPublished - 2010 Jul
Externally publishedYes

Keywords

  • 5-HT
  • CCK
  • Membrane/nuclear
  • Neuropetides
  • Noradrenaline
  • Receptors
  • Vasopressin

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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