Neuroprotective effects of focal brain cooling on photochemically-induced cerebral infarction in rats: Analysis from a neurophysiological perspective

Yeting He, Masami Fujii, Takao Inoue, Sadahiro Nomura, Yuichi Maruta, Fumiaki Oka, Satoshi Shirao, Yuji Owada, Hiroyuki Kida, Ichiro Kunitsugu, Toshitaka Yamakawa, Tatsuji Tokiwa, Takeshi Yamakawa, Michiyasu Suzuki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Although systemic hypothermia provides favorable outcomes in stroke patients, it has only been adopted in a limited number of patients because of fatal complications. To resolve these issues, focal brain cooling (FBC) has recently drawn attention as a less-invasive treatment for brain injuries. Therefore, we investigated whether FBC has a favorable effect on focal cerebral ischemia (FCI). Male-adult-Wistar rats were used. Under general anesthesia, a small burr hole was made and FCI was induced in the primary sensorimotor area (SI-MI) using photothrombosis. An additional craniotomy was made over the SI-MI and FBC was performed at a temperature of 15 °C for 5 h. Electrocorticograms (ECoG) were recorded on the border cortex of the ischemic focus. Thereafter, rats were sacrificed and the infarct area was measured. In another experiment, rats were allowed to recover for 5 days after cooling and neurobehavioral function was evaluated. FBC suppressed all ECoG frequency bands during and after cooling (p<0.05), except for the delta frequency band in the precooling versus rewarming periods. The injured areas in the cooling and non-cooling groups were 0.99±0.30 and 1.71±0.54 mm2, respectively (p<0.03). The grip strength at 2 days after surgery was preserved in the cooling group (p<0.05). We report the novel finding that epileptiform discharges were suppressed in the ischemic border, the infarct area was reduced and neurobehaviour was preserved by FBC. These results indicate that FBC is neuroprotective in the ischemic brain and has demonstrated therapeutic potential for cerebral infarction.

Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalBrain research
Volume1497
DOIs
Publication statusPublished - 2013 Feb 25
Externally publishedYes

Keywords

  • Epileptiform discharge
  • Focal brain cooling
  • Neurobehavior
  • Photothrombotic cerebral infarction
  • Triphenyltetrazolium chloride (TTC)

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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  • Cite this

    He, Y., Fujii, M., Inoue, T., Nomura, S., Maruta, Y., Oka, F., Shirao, S., Owada, Y., Kida, H., Kunitsugu, I., Yamakawa, T., Tokiwa, T., Yamakawa, T., & Suzuki, M. (2013). Neuroprotective effects of focal brain cooling on photochemically-induced cerebral infarction in rats: Analysis from a neurophysiological perspective. Brain research, 1497, 53-60. https://doi.org/10.1016/j.brainres.2012.11.041