Neuroprotective effect against axonal damage-induced retinal ganglion cell death in apolipoprotein E-deficient mice through the suppression of kainate receptor signaling

Kazuko Omodaka, Koji M. Nishiguchi, Masayuki Yasuda, Yuji Tanaka, Kota Sato, Orie Nakamura, Kazuichi Maruyama, Toru Nakazawa

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Apolipoprotein E (ApoE) plays important roles in the body, including a carrier of cholesterols, an anti-oxidant, and a ligand for the low-density lipoprotein receptors. In the nervous system, the presence of ApoE4 isoforms is associated with Alzheimers disease. ApoE gene polymorphisms are also associated with glaucoma, but the function of ApoE in the retina remains unclear. In this study, we investigated the role of ApoE in axonal damage-induced RGC death. ApoE was detected in the astrocytes and Müller cells in the wild-type (WT) retina. RGC damage was induced in adult ApoE-deficient mice (male, 10-12 weeks old) through ocular hypertension (OH), optic nerve crush (NC), or by administering kainic acid (KA) intravitreally. The WT mice were treated with a glutamate receptor antagonist (MK801 or CNQX) 30 min before performing NC or left untreated. Seven days later, the retinas were flat mounted and Fluorogold-labeled RGCs were counted. We found that the RGCs in the ApoE-deficient mice were resistant to OH-induced RGC death and optic nerve degeneration 4 weeks after induction. In WT mice, NC effectively induced RGC death (control: 4085±331 cells/mm2, NC: 1728±170 cells/mm2). CNQX, an inhibitor of KA receptors, suppressed this RGC death (3031±246 cells/mm2), but MK801, an inhibitor of NMDA receptors, did not (1769±212 cells/mm2). This indicated the involvement of KA receptor signaling in NC-induced RGC death. We found that NC- or KA-induced RGC death was significantly less in the ApoE-deficient mice than in the WT mice. These data suggest that the ApoE deficiency had a neuroprotective effect against axonal damage-induced RGC death by suppressing the KA receptor signaling.

Original languageEnglish
Pages (from-to)203-212
Number of pages10
JournalBrain research
Volume1586
DOIs
Publication statusPublished - 2014 Oct 24

Keywords

  • Astrocyte
  • Excitotoxicity
  • Glaucoma
  • Optic nerve
  • Retinal ganglion cell

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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