Neurophilin-1 is a downstream target of transcription factor Ets-1 in human umbilical vein endothelial cells

Kazuhide Teruyama, Mayumi Abe, Toru Nakano, Shoki Takahashi, Shogo Yamada, Yasufumi Sato

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Transcription factor Ets-1 expressed in endothelial cells promotes angiogenesis. Here, we transiently overexpressed Ets-1 in human umbilical vein endothelial cells (HUVECs) and comprehensively searched for potential downstream targets of Ets-1 by cDNA microarray analysis. The expression of several angiogenesis-related genes including neuropilin-1 was augmented by the overexpression of Ets-1. Quantitative real-time RT-PCR and Western blotting confirmed the increase in the levels of neuropilin-1 mRNA and protein. In contrast, dominant negative ets-1 decreased the levels of neuropilin-1 mRNA and protein. These results indicate that neuropilin-1 is a downstream target of Ets-1 in HUVECs.

Original languageEnglish
Pages (from-to)1-4
Number of pages4
JournalFEBS Letters
Volume504
Issue number1-2
DOIs
Publication statusPublished - 2001 Aug 24

Keywords

  • Angiogenesis
  • Endothelial cell
  • Ets-1
  • Neuropilin-1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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