Neuropeptide Y inhibits neurogenic inflammation in guinea pig airways

T. Takahashi, M. Ichinose, H. Yamauchi, M. Miura, N. Nakajima, J. Ishikawa, H. Inoue, T. Takishima, K. Shirato

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

We examined the effect of neuropeptide Y (NPY) on neurogenic airway microvascular leakage. Male Dunkin-Hartley guinea pigs (250-350 g) were anesthetized with urethan (2 g/kg ip). The cervical artery and vein were cannulated for monitoring blood pressure and injecting drugs, respectively. Atropine and propranolol (each 1 mg/kg iv) were administered 30 min before the experiment. After pretreatment with saline (vehicle for NPY) or NPY (1- 100 μg/kg iv), Evans blue dye (30 mg/kg iv) was administered. Then, bilateral vagal nerves were electrically stimulated (5 V, 7 Hz, 5-ms duration for 3 min) to induce airway plasma leakage. Airways were divided into four sections [trachea (Tr), main bronchi, central intrapulmonary airways (IPA), and peripheral IPA] and incubated in formamide (37°C for 16 h). The concentration of Evans blue dye was measured by spectrophotometer. Furthermore, we examined the effect of NPY on exogenous substance P- (0.3 μg/kg iv) induced plasma extravasation. Bilateral vagal stimulation significantly increased leakage of dye in Tr to peripheral IPA. NPY did not affect basal leakage but did significantly inhibit neurogenic plasma extravasation in a dose-dependent manner with maximal inhibitions of 42.3 (Tr), 67.7 (main bronchi), 38.2 (central IPA), and 26.3% (peripheral IPA) at 30 μg/kg. Exogenous substance P-induced plasma extravasation was not inhibited by NPY. We conclude that NPY inhibits neurogenic inflammation by prejunctional inhibition of neuropeptide release from airway sensory nerve terminals.

Original languageEnglish
Pages (from-to)103-107
Number of pages5
JournalJournal of Applied Physiology
Volume75
Issue number1
DOIs
Publication statusPublished - 1993

Keywords

  • airway permeability
  • asthma
  • potassium channel
  • prejunctional modulation
  • substance P

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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