Neuropeptide substance P stimulates the formation of osteoclasts via synovial fibroblastic cells

Takaaki Matayoshi, Tetsuya Goto, Eiji Fukuhara, Hiroshi Takano, Shigeru Kobayashi, Tetsu Takahashi

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

The present study was designed to evaluate the effects of neuropeptide substance P (SP) on the formation of osteoclasts via synovial fibroblastic cells. Synovial fibroblastic cells derived from rat knee joint expressed the SP receptor, neurokinin-1 receptor (NK 1-R). The addition of SP stimulated the proliferation of synovial fibroblastic cells and this effect was inhibited by SP or NK 1-R antagonists. Increased expression of the receptor activator of nuclear factor κB ligand (RANKL) in synovial fibroblastic cells after the addition of SP was demonstrated by reverse transcriptase-polymerase chain reaction and immunofluorescence staining. Osteoprotegerin expression in synovial fibroblastic cells was decreased after incubation with SP. In co-cultures of synovial fibroblastic cells and rat peripheral blood monocytes, SP stimulated osteoclastogenesis. These results suggest that SP in the joint cavity may cause both hypertrophy of the synovium and induction of increased osteoclast formation through the increased expression of RANKL in the synovium.

Original languageEnglish
Pages (from-to)756-764
Number of pages9
JournalBiochemical and biophysical research communications
Volume327
Issue number3
DOIs
Publication statusPublished - 2005 Feb 18

Keywords

  • Neurokinin-1 receptor
  • Osteoclastogenesis
  • Osteoprotegerin
  • Receptor activator of nuclear factor κB ligand
  • Substance P
  • Synovial fibroblastic cells

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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