Abstract
An in situ approach was used to identify the oxidized RNA nucleoside 8-hydroxyguanosine (8OHG) in the frontal cortex of familial Alzheimer's disease (FAD) with a mutation in presenilin-1 (PS-1) or amyloid β protein precursor (AβPP) gene (n = 13, age 47-81 years). Neurons with marked 8OHG immunoreaction in the cytoplasm were widely distributed in the superior/middle frontal gyrus of FAD. Relative intensity measurements of neuronal 8OHG immunoreactivity showed that there was a significant increase in FAD compared with controls (n = 15, age 59-81 years), while there was no difference in relative 8OHG between the PS-1 and the AβPP FAD. Interestingly, a presymptomatic case carrying a PS-1 mutation showed a considerable level of relative 8OHG, and the increased levels of neuronal 8OHG in FAD were more prominent in cases with a lower percentage area of Aβ42 burden. These results suggest that oxidative stress is an early event involved in the pathological cascade of FAD.
Original language | English |
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Pages (from-to) | 108-113 |
Number of pages | 6 |
Journal | Neurobiology of Disease |
Volume | 17 |
Issue number | 1 |
DOIs | |
Publication status | Published - 2004 Oct |
Externally published | Yes |
Keywords
- 8-Hydroxyguanosine
- Amyloid β protein precursor
- Familial Alzheimer's disease
- Oxidative stress
- Presenilin
- RNA
ASJC Scopus subject areas
- Neurology