Neuronal RNA oxidation is a prominent feature of dementia with Lewy bodies

Akihiko Nunomura, Shigeru Chiba, Kenji Kosaka, Atsushi Takeda, Rudy J. Castellani, Mark A. Smith, George Perry

Research output: Contribution to journalArticlepeer-review

63 Citations (Scopus)

Abstract

An in situ approach was used to identify the oxidized nucleoside, 8-hydroxyguanosine in brains of dementia with Lewy bodies. Neurons with marked immunoreaction of 8-hydroxyguanosine in the cytoplasm were widely distributed in the hippocampal region and temporal neocortex. Relative intensity measurements of neuronal 8-hydroxyguanosine immunoreactivity showed that there was a significant increase in nucleic acid oxidation in dementia with Lewy bodies compared with controls. Treatment with nuclease (DNase or RNase) before the immunostaining demonstrated that RNA was a major site of nucleic acid oxidation. Together with the previously reported RNA oxidation in vulnerable neurons in Alzheimer and Parkinson diseases, neuronal RNA oxidation in dementia with Lewy bodies might represent one of the fundamental abnormalities in age-associated neurodegenerative diseases.

Original languageEnglish
Pages (from-to)2035-2039
Number of pages5
JournalNeuroReport
Volume13
Issue number16
DOIs
Publication statusPublished - 2002 Nov 15

Keywords

  • 8-Hydroxyguanosine
  • Dementia with Lewy bodies
  • Oxidative stress
  • RNA
  • Synucleinopathy

ASJC Scopus subject areas

  • Neuroscience(all)

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