@article{d2bc5d18296640108faaecd3d905e81e,
title = "Neuronal octopamine signaling regulates mating-induced germline stem cell increase in female Drosophila melanogaster",
abstract = "Stem cells fuel the development and maintenance of tissues. Many studies have addressed how local signals from neighboring niche cells regulate stem cell identity and their proliferative potential. However, the regulation of stem cells by tissue-extrinsic signals in response to environmental cues remains poorly understood. Here we report that efferent octopaminergic neurons projecting to the ovary are essential for germline stem cell (GSC) increase in response to mating in female Drosophila. The neuronal activity of the octopaminergic neurons is required for mating-induced GSC increase as they relay the mating signal from sex peptide receptor-positive cholinergic neurons. Octopamine and its receptor Oamb are also required for mating-induced GSC increase via intracellular Ca2+ signaling. Moreover, we identified Matrix metalloproteinase-2 as a downstream component of the octopamine-Ca2+ signaling to induce GSC increase. Our study provides a mechanism describing how neuronal system couples stem cell behavior to environmental cues through stem cell niche signaling.",
author = "Yuto Yoshinari and Tomotsune Ameku and Shu Kondo and Hiromu Tanimoto and Takayuki Kuraishi and Yuko Shimada-Niwa and Ryusuke Niwa",
note = "Funding Information: We thank Toshiro Aigaki, Kendal S Broadie, Aki Ejima, Kyung-An Han, Yukako Hattori, Yoshiki Hayashi, Makoto Ihara, Young-Joon Kim, Satoru Kobayashi, Kazuhiko Matsuda, Masayuki Miura, Akira Nakamura, Takashi Nishimura, Andrea Page-McCaw, Hiroko Sano, Jianjun Sun, Nobuaki Tanaka, James W Truman, Tadashi Uemura, Daisuke Yamamoto, the Bloomington Stock Center, the Kyoto Stock Center (DGRC), the National Institute of Genetics, the Vienna Drosophila Resource Center, and the Developmental Studies Hybridoma Bank for providing stocks and reagents; Aki Hori and Reiko Kise for their technical assistance; and Satoru Kobayashi and Shosei Yoshida for their helpful discussion. YY and TA were recipients of the fellowship from the Japan Society for the Promotion of Science. This work was supported by grants from KAKENHI (19H05240 to RN, 26250001 and 17H01378 to HT, 18J20572 to YY, and 15J00652 to TA), AMED-PRIME, AMED (17gm6010011h0001 to TK), AMED-CREST, AMED (19gm1110001h0003 to RN), and the Takeda Science Foundation to RN. Funding Information: We thank Toshiro Aigaki, Kendal S Broadie, Aki Ejima, Kyung-An Han, Yukako Hattori, Yoshiki Haya-shi, Makoto Ihara, Young-Joon Kim, Satoru Kobayashi, Kazuhiko Matsuda, Masayuki Miura, Akira Nakamura, Takashi Nishimura, Andrea Page-McCaw, Hiroko Sano, Jianjun Sun, Nobuaki Tanaka, James W Truman, Tadashi Uemura, Daisuke Yamamoto, the Bloomington Stock Center, the Kyoto Stock Center (DGRC), the National Institute of Genetics, the Vienna Drosophila Resource Center, and the Developmental Studies Hybridoma Bank for providing stocks and reagents; Aki Hori and Reiko Kise for their technical assistance; and Satoru Kobayashi and Shosei Yoshida for their helpful discussion. YY and TA were recipients of the fellowship from the Japan Society for the Promotion of Science. This work was supported by grants from KAKENHI (19H05240 to RN, 26250001 and 17H01378 to HT, 18J20572 to YY, and 15J00652 to TA), AMED-PRIME, AMED (17gm6010011h0001 to TK), AMED-CREST, AMED (19gm1110001h0003 to RN), and the Takeda Science Foundation to RN. Publisher Copyright: {\textcopyright} Yoshinari et al.",
year = "2020",
month = oct,
doi = "10.7554/eLife.57101",
language = "English",
volume = "9",
pages = "1--35",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}