Neural tube defects and impaired neural progenitor cell proliferation in Gβ1-deficient mice

Hiroaki Okae, Yoichiro Iwakura

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Heterotrimeric G proteins are well known for their roles in signal transduction downstream of G protein-coupled receptors (GPCRs), and both Gα subunits and tightly associated Gβγ subunits regulate downstream effector molecules. Compared to Gα subunits, the physiological roles of individual Gβ and Gγ subunits are poorly understood. In this study, we generated mice deficient in the Gβ1 gene and found that Gβ1 is required for neural tube closure, neural progenitor cell proliferation, and neonatal development. About 40% Gβ1-/- embryos developed neural tube defects (NTDs) and abnormal actin organization was observed in the basal side of neuroepithelium. In addition, Gβ1-/- embryos without NTDs showed microencephaly and died within 2 days after birth. GPCR agonist-induced ERK phosphorylation, cell proliferation, and cell spreading, which were all found to be regulated by Gαi and Gβγ signaling, were abnormal in Gβ1-/- neural progenitor cells. These data indicate that Gβ1 is required for normal embryonic neurogenesis.

Original languageEnglish
Pages (from-to)1089-1101
Number of pages13
JournalDevelopmental Dynamics
Volume239
Issue number4
DOIs
Publication statusPublished - 2010 Apr 1
Externally publishedYes

Keywords

  • Actin cytoskeleton
  • ERK
  • G protein-coupled receptor
  • Heterotrimeric G protein
  • Neural progenitor cell
  • Neural tube defect

ASJC Scopus subject areas

  • Developmental Biology

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