Neural crest-derived multipotent cells in the adult mouse iris stroma

Miki Kikuchi, Ryuhei Hayashi, Sachiko Kanakubo, Ayumi Ogasawara, Masayuki Yamato, Noriko Osumi, Kohji Nishida

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The purpose of this study was to characterize neural crest-derived cells within the adult murine iris. The iris was isolated from P0-Cre/Floxed-EGFP transgenic (TG) mice. The isolated iris cells formed EGFP-positive spheres on non-adhesive culture plates. Immunostaining showed that these EGFP-positive spheres expressed neural crest markers including Sox10 and p75NTR, and these cells showing in vitro sphere-forming ability were originally resided in the iris stroma (IS), in vivo. Real-time RT-PCR showed that the EGFP-positive spheres expressed significantly higher levels of the neural crest markers than EGFP-negative spheres and bone marrow-derived mesenchymal stem cells. Furthermore, the iris stromal sphere had capability to differentiate into various cell lineages including smooth muscle and cartilage. These data indicate that neural crest-derived multipotent cells can be isolated from the murine IS and expanded in sphere culture.

Original languageEnglish
Pages (from-to)273-281
Number of pages9
JournalGenes to Cells
Volume16
Issue number3
DOIs
Publication statusPublished - 2011 Mar 1

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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    Kikuchi, M., Hayashi, R., Kanakubo, S., Ogasawara, A., Yamato, M., Osumi, N., & Nishida, K. (2011). Neural crest-derived multipotent cells in the adult mouse iris stroma. Genes to Cells, 16(3), 273-281. https://doi.org/10.1111/j.1365-2443.2011.01485.x