Nerve elements containing met-enkephalin-Arg6-Gly7-Leu8-like immunoreactivity in canine pancreas - A histochemical study

Tooru Shimosegawa, Shigeru Kobayashi, Tsuneo Fujita, Tooru Mochizuki, Chizuko Yanaihara, Noboru Yanaihara

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13 Citations (Scopus)


Canine pancreas was examined by Sternberger's peroxidase-antiperoxidase method using an antiserum against Met-enkephalin-Arg6-Gly7-Leu8 (Met-Enk-Arg-Gly-Leu), an opioid octapeptide included in preproenkephalin A. Immunoreactive Met-Enk-Arg-Gly-Leu-containing nerve terminals with beaded appearance were demonstrated to be juxtaposed to small vessels of the exocrine and endocrine pancreas. Eighty-five out of 174 ganglion cell somas in the pancreas were immunopositive. The specific immunostaining was absorbed by preincubation of the antiserum (dilution, 1:2000) with Met-Enk-Arg-Gly-Leu (50 μg/ml), but not with Leu-enkephalin and Met-enkephalin (100 μg/ml).

Original languageEnglish
Pages (from-to)161-165
Number of pages5
JournalNeuroscience Letters
Issue number2
Publication statusPublished - 1983 Dec 2


  • Met-enkephalin-Arg-Gly-Leu
  • dog
  • immunohistochemistry
  • neuroinsular complex
  • pancreas
  • pancreatic islet
  • preproenkephalin A

ASJC Scopus subject areas

  • Neuroscience(all)


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