Neospora caninum: Application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice

Houshuang Zhang, Yoshifumi Nishikawa, Junya Yamagishi, Jinlin Zhou, Yuzuru Ikehara, Naoya Kojima, Naoaki Yokoyama, Xuenan Xuan

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (M3-DPPE), referred to as M3-DPPE liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. To evaluate whether these M3-DPPE liposomes have an adjuvant capacity against Neospora caninum infection, a novel immunization method utilizing soluble N. caninum apical membrane antigen 1 (NcAMA1) encapsulated in the M3-DPPE liposomes (M3-NcAMA1) was employed. The results revealed that a significant amount of interferon (IFN)-γ production was detected in culture supernatants of NcAMA1 protein- or N. caninum lysate-stimulated spleen cells obtained from the mice one week after the third immunization with M3-NcAMA1. The parasite burden in the dams' brain tissue was decreased and the survival rate of offspring increased significantly in M3-NcAMA1-immunized mice. Thus, a parasite-specific Th1 immune response was successfully induced in the pregnant mice immunized with M3-NcAMA1, and an effective reduction of offspring mortality from N. caninum infection was triggered.

    Original languageEnglish
    Pages (from-to)130-136
    Number of pages7
    JournalExperimental Parasitology
    Volume125
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jun

    Keywords

    • Apical membrane antigen 1
    • Neospora caninum
    • Oligomannose-coated liposomes

    ASJC Scopus subject areas

    • Parasitology
    • Immunology
    • Infectious Diseases

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