Neoechinulin a imparts resistance to acute nitrosative stress in PC12 cells: A potential link of an elevated cellular reserve capacity for pyridine nucleotide redox turnover with cytoprotection

Soichiro Akashi, Kyo Shirai, Tatsumi Okada, Kanako Konishi, Toshifumi Takeuchi, Kouji Kuramochi, Muneaki Takahashi, Tomoya Nakagawa, Yuki Ogura, Satoshi Fujieda, Yasushi Shibata, Fumio Sugawara, Susumu Kobayashi, Nobuo Watanabe, Takao Araia

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Treatment of PC12 cells with fungus-derived alkaloid neoechinulin A for more than 12 h renders the cells resistant to subsequent superoxide (O 2-)/nitric oxide (NO) insults derived from 3-morpholinosydnonimine (SIN-1). However, the underlying mechanism(s) remains largely unclear. To elucidate the mechanism(s), we assessed the specificity of the cytoprotection afforded by neoechinulin A treatment using other cytocidal stressors and also clarified the resulting cellular alterations, focusing on the antioxidant and metabolic enzymes systems. Neoechinulin A treatment for more than 12 h endowed PC12 cells with significant resistance to transient NO toxicity, but not persistent NO toxicity, bolus H2O2 toxicity, or oxidative insult from the redox cycling quinone menadione. Cellular antioxidant system profiling revealed no substantial potentiation of the activity of any antioxidant enzyme in lysate from the neoechinulin A-treated cells excluding glutathione (GSH) content, which was significantly decreased (>50%), resulting in a proportional compromise in the thiol-reducing activity of the intact cells. In addition, no differences were observed in the activity for any nicotinamide adenine dinucleotide (phosphate) reduced form (NAD(P)H)-generating enzyme, steady-state NAD(P)H/nicotinamide adenine dinucleotide (phosphate) oxidized form (NAD(P)+) ratios, or the levels of total NAD(P)H. Nevertheless, the neoechinulin A-treated intact cells exhibited increased NAD(P)H redox turnover when driven by extracellular tetrazolium. The structurally inactive analog preechinulin failed to protect cells against NO toxicity or induce these alterations, suggesting their link with the cytoprotective mechanism. These results suggest that neoechinulin A, despite disabling the GSH defense system, confers cytoprotection against nitrosative stresses by elevating the cellular reserve capacity for NAD(P)H generation, which could offset crippling of energy-supplying systems due to nitrosative stress.

Original languageEnglish
Pages (from-to)1105-1117
Number of pages13
JournalBiological and Pharmaceutical Bulletin
Volume35
Issue number7
DOIs
Publication statusPublished - 2012 Jul

Keywords

  • 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium
  • Glutathione
  • Neurodegeneration
  • Nitrosative stress
  • Reserve capacity

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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