Nek5, a novel substrate for caspase-3, promotes skeletal muscle differentiation by up-regulating caspase activity

Kouhei Shimizu, Tatsuya Sawasaki

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Accumulating evidence suggests that caspase-3-mediated cleavage of protein kinase could be a key event to regulate cell differentiation. In this study, we investigated the role of Nek5 kinase, identified as a novel substrate for caspase-3, in skeletal muscle differentiation. Up-regulation of Nek5 mRNA expression was accompanied by cell differentiation. Myotube formation was promoted in Nek5 expressing cells, and was conversely inhibited in Nek5 knockdown cells. Furthermore, we found that caspase-3 activity, an important factor for myogenic differentiation, was enhanced by Nek5 cleavage. Although caspase-3-cleaved Nek5 partially exerted a promyogenic effect, it tended to induce apoptotic cell death. In summary, our findings suggest that Nek5 promotes myogenic differentiation through up-regulation of caspase activity.

Original languageEnglish
Pages (from-to)2219-2225
Number of pages7
JournalFEBS Letters
Volume587
Issue number14
DOIs
Publication statusPublished - 2013 Jul 11

Keywords

  • C2C12 myoblast
  • Caspase-3
  • Muscle differentiation
  • Nek5

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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