TY - JOUR
T1 - Nek5, a novel substrate for caspase-3, promotes skeletal muscle differentiation by up-regulating caspase activity
AU - Shimizu, Kouhei
AU - Sawasaki, Tatsuya
N1 - Funding Information:
The authors would like to acknowledge Dr. Atsuko Sehara (Kyoto University) for kindly providing the C2/4 (C2C12) cells. This work was supported by a Grant-in-Aid for Scientific Research (B) (T.S.) and a Grant-in-Aid for JSPS Fellows (K.S.) from Japan Society for the Promotion of Science , and a Grant-in-Aid for Scientific Research on Innovative Areas (T.S.) from the Ministry of Education, Culture, Sports, Science, and Technology in Japan .
PY - 2013/7/11
Y1 - 2013/7/11
N2 - Accumulating evidence suggests that caspase-3-mediated cleavage of protein kinase could be a key event to regulate cell differentiation. In this study, we investigated the role of Nek5 kinase, identified as a novel substrate for caspase-3, in skeletal muscle differentiation. Up-regulation of Nek5 mRNA expression was accompanied by cell differentiation. Myotube formation was promoted in Nek5 expressing cells, and was conversely inhibited in Nek5 knockdown cells. Furthermore, we found that caspase-3 activity, an important factor for myogenic differentiation, was enhanced by Nek5 cleavage. Although caspase-3-cleaved Nek5 partially exerted a promyogenic effect, it tended to induce apoptotic cell death. In summary, our findings suggest that Nek5 promotes myogenic differentiation through up-regulation of caspase activity.
AB - Accumulating evidence suggests that caspase-3-mediated cleavage of protein kinase could be a key event to regulate cell differentiation. In this study, we investigated the role of Nek5 kinase, identified as a novel substrate for caspase-3, in skeletal muscle differentiation. Up-regulation of Nek5 mRNA expression was accompanied by cell differentiation. Myotube formation was promoted in Nek5 expressing cells, and was conversely inhibited in Nek5 knockdown cells. Furthermore, we found that caspase-3 activity, an important factor for myogenic differentiation, was enhanced by Nek5 cleavage. Although caspase-3-cleaved Nek5 partially exerted a promyogenic effect, it tended to induce apoptotic cell death. In summary, our findings suggest that Nek5 promotes myogenic differentiation through up-regulation of caspase activity.
KW - C2C12 myoblast
KW - Caspase-3
KW - Muscle differentiation
KW - Nek5
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U2 - 10.1016/j.febslet.2013.05.049
DO - 10.1016/j.febslet.2013.05.049
M3 - Article
C2 - 23727203
AN - SCOPUS:84879691440
VL - 587
SP - 2219
EP - 2225
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 14
ER -