Abstract
We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 μg/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.
Original language | English |
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Pages (from-to) | 275-279 |
Number of pages | 5 |
Journal | Biochemical and biophysical research communications |
Volume | 244 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1998 Mar 6 |
Keywords
- Botulinum toxin
- Cardiac myocytes
- Chronotropic effect
- Clostridium botulinum
- SNAP-25
- SNARE
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology