Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes

Koichi Kimura; Hisakazu Kimura; Noriko Yokosawa; Hiroshi Isogai; Emiko Isogai; Shunji Kozaki; Atsushi Miyamoto; Takeshi Nishikawa; Hideyo Ohshika; Toru Kubota; Nobuhiro Fujii

doi:10.1006/bbrc.1998.8188

Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes

Koichi Kimura, Hisakazu Kimura, Noriko Yokosawa, Hiroshi Isogai, Emiko Isogai, Shunji Kozaki, Atsushi Miyamoto, Takeshi Nishikawa, Hideyo Ohshika, Toru Kubota, Nobuhiro Fujii

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 μg/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.

Original language	English
Pages (from-to)	275-279
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	244
Issue number	1
DOIs	https://doi.org/10.1006/bbrc.1998.8188
Publication status	Published - 1998 Mar 6
Externally published	Yes

Keywords

Botulinum toxin
Cardiac myocytes
Chronotropic effect
Clostridium botulinum
SNAP-25
SNARE

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/bbrc.1998.8188

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Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes. / Kimura, Koichi; Kimura, Hisakazu; Yokosawa, Noriko; Isogai, Hiroshi; Isogai, Emiko; Kozaki, Shunji; Miyamoto, Atsushi; Nishikawa, Takeshi; Ohshika, Hideyo; Kubota, Toru; Fujii, Nobuhiro.

In: Biochemical and biophysical research communications, Vol. 244, No. 1, 06.03.1998, p. 275-279.

Research output: Contribution to journal › Article › peer-review

Kimura, Koichi ; Kimura, Hisakazu ; Yokosawa, Noriko ; Isogai, Hiroshi ; Isogai, Emiko ; Kozaki, Shunji ; Miyamoto, Atsushi ; Nishikawa, Takeshi ; Ohshika, Hideyo ; Kubota, Toru ; Fujii, Nobuhiro. / Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes. In: Biochemical and biophysical research communications. 1998 ; Vol. 244, No. 1. pp. 275-279.

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abstract = "We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 μg/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.",

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AU - Kozaki, Shunji

AU - Miyamoto, Atsushi

AU - Nishikawa, Takeshi

AU - Ohshika, Hideyo

AU - Kubota, Toru

AU - Fujii, Nobuhiro

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AB - We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 μg/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.

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Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes

Abstract

Keywords

ASJC Scopus subject areas

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