Negative chronotropic effect of botulinum toxin on neonatal rat cardiac myocytes

Koichi Kimura, Hisakazu Kimura, Noriko Yokosawa, Hiroshi Isogai, Emiko Isogai, Shunji Kozaki, Atsushi Miyamoto, Takeshi Nishikawa, Hideyo Ohshika, Toru Kubota, Nobuhiro Fujii

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


We demonstrated that botulinum neurotoxin attenuated the spontaneous beating rate of cultured cardiac myocytes. Primary cultured cardiac myocytes were prepared from the ventricles of neonatal Wistar rats (1-3 days old). On 7 days after cell seeding, botulinum toxin type A incorporated into liposomes was added to the culture medium. At a final concentration of 5.0 μg/ml, botulinum toxin markedly attenuated the beating rate of cardiac myocytes within 2-4 hours. These results demonstrated the effect of SNARE-complex proteins on the spontaneous beating of cardiac myocytes.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalBiochemical and biophysical research communications
Issue number1
Publication statusPublished - 1998 Mar 6
Externally publishedYes


  • Botulinum toxin
  • Cardiac myocytes
  • Chronotropic effect
  • Clostridium botulinum
  • SNAP-25

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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