Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein

Kenichi Takahashi, Hiroyuki Nakanishi, Masako Miyahara, Kenji Mandai, Keiko Satoh, Ayako Satoh, Hideo Nishioka, Junken Aoki, Akio Nomoto, Akira Mizoguchi, Yoshimi Takai

Research output: Contribution to journalArticle

414 Citations (Scopus)

Abstract

We have isolated a novel actin filament-binding protein, named afadin, localized at cadherin-based cell-cell adherens junctions (AJs) in various tissues and cell lines. Afadin has one PDZ domain, three proline-rich regions, and one actin filament-binding domain. We found here that afadin directly interacted with a family of the immunoglobulin superfamily, which was isolated originally as the poliovirus receptor-related protein (PRR) family consisting of PRR1 and -2, and has been identified recently to be the alphaherpes virus receptor. PRR has a COOH-terminal consensus motif to which the PDZ domain of afadin binds. PRR and afadin were colocalized at cadherin- based cell-cell AJs in various tissues and cell lines. In E-cadherin- expressing EL cells, PRR was recruited to cadherin-based cell-cell AJs through interaction with afadin. PRR showed Ca2+-independent cell-cell adhesion activity. These results indicate that PRR is a cell-cell adhesion molecule of the immunoglobulin superfamily which is recruited to cadherin- based cell-cell AJs through interaction with afadin. We rename PRR as nectin (taken from the Latin word 'necto' meaning 'to connect').

Original languageEnglish
Pages (from-to)539-549
Number of pages11
JournalJournal of Cell Biology
Volume145
Issue number3
DOIs
Publication statusPublished - 1999 May 3

Keywords

  • Afadin
  • Cadherin
  • Immunoglobulin superfamily
  • Poliovirus receptor-related protein
  • Zonula adherens

ASJC Scopus subject areas

  • Cell Biology

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    Takahashi, K., Nakanishi, H., Miyahara, M., Mandai, K., Satoh, K., Satoh, A., Nishioka, H., Aoki, J., Nomoto, A., Mizoguchi, A., & Takai, Y. (1999). Nectin/PRR: An immunoglobulin-like cell adhesion molecule recruited to cadherin-based adherens junctions through interaction with afadin, a PDZ domain-containing protein. Journal of Cell Biology, 145(3), 539-549. https://doi.org/10.1083/jcb.145.3.539