Near-field scanning optical microscopy studies of L-α- dipalmitoylphosphatidylcholine monolayers at the air-liquid interface

H. Shiku, R. C. Dunn

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The phase structure in L-α-dipalmitoylphosphatidylcholine-2.0 mol% fluorescent 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate Langmuir monolayers dispersed on a 2 M sucrose solution subphase is studied with near-field scanning optical microscopy (NSOM). Cantilevered NSOM probes operating in a tapping-mode feedback or an optical interferometric feedback mode are capable of tracking the air-sucrose solution interface. At the micrometre scale, the NSOM fluorescence images reveal lipid domain features similar to those observed previously in supported Langmuir-Blodgett (LB) monolayers. At the submicrometre scale, the small nanometric lipid islands seen in LB films are not observed at the air- sucrose interface. This supports a mechanism in which domain formation in LB films can be induced by means of the transfer process onto the solid support. Progress towards extending these studies to films at the air-water interface using the optical interferometric feedback method is also discussed.

Original languageEnglish
Pages (from-to)461-466
Number of pages6
JournalJournal of Microscopy
Volume194
Issue number2-3
DOIs
Publication statusPublished - 1999 Jan 1
Externally publishedYes

Keywords

  • Air-liquid interface
  • DPPC monolayer
  • Near-field scanning optical microscopy
  • Phase transition
  • Sucrose solution

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

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