@article{54597cc8d1fe47a79af38c7af4be9a5c,
title = "NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells",
abstract = "Abstract NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas.",
keywords = "Calpain, Cell cycle, MYCN, Myc-nick, NCYM, Neuroblastoma",
author = "Wataru Shoji and Yusuke Suenaga and Yoshiki Kaneko and Islam, {S. M.Rafiqul} and Jennifer Alagu and Sana Yokoi and Masaki Nio and Akira Nakagawara",
note = "Funding Information: We thank Y. Nakamura, Y. Tatsumi, A. Takatori, E. Isogai, and T. Yokochi for comments and advice. This work was supported in part by a Grant-in-Aid from the Ministry of Health, Labour and Welfare for the Third Term Comprehensive Control Research for Cancer, Japan (AN), a Grant-in-Aid for Scientific Research on Priority Areas (JSPS KAKENHI Grant Number 17015046 ) (AN), a Grant-in-Aid for Scientific Research (A) (JSPS KAKENHI Grant Number 24249061 ) (AN), a Grant-in-Aid for Research Activity start-up (JSPS KAKENHI Grant Number 22890241 ) (YS) and a Grant-in-Aid for Young Scientists (B) (JSPS KAKENHI Grant Number 24700957 ) (YS) from JSPS. Funding Information: We report grants from the Ministry of Health, Labour and Welfare for the Third Term Comprehensive Control Research for Cancer, Japan (AN), grants from the Japan Society for the Promotion of Science (JSPS) (YS and AN), during the conduct of the study; grants from Takeda Science Foundation (SY and AN), grants from GlaxoSmithKline, outside the submitted work; In addition, YS and AN have a patent neuroblastoma mouse model pending. This does not alter the authors' adherence to all policies of Biochemical and Biophysical Research Communications. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Publisher Copyright: {\textcopyright} 2015 The Authors.",
year = "2015",
month = jun,
day = "12",
doi = "10.1016/j.bbrc.2015.04.050",
language = "English",
volume = "461",
pages = "501--506",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "3",
}