NCYM promotes calpain-mediated Myc-nick production in human MYCN-amplified neuroblastoma cells

Wataru Shoji, Yusuke Suenaga, Yoshiki Kaneko, S. M.Rafiqul Islam, Jennifer Alagu, Sana Yokoi, Masaki Nio, Akira Nakagawara

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

Abstract NCYM is a cis-antisense gene of MYCN and is amplified in human neuroblastomas. High NCYM expression is associated with poor prognoses, and the NCYM protein stabilizes MYCN to promote proliferation of neuroblastoma cells. However, the molecular mechanisms of NCYM in the regulation of cell survival have remained poorly characterized. Here we show that NCYM promotes cleavage of MYCN to produce the anti-apoptotic protein, Myc-nick, both in vitro and in vivo. NCYM and Myc-nick were induced at G2/M phase, and NCYM knockdown induced apoptotic cell death accompanied by Myc-nick downregulation. These results reveal a novel function of NCYM as a regulator of Myc-nick production in human neuroblastomas.

Original languageEnglish
Article number33796
Pages (from-to)501-506
Number of pages6
JournalBiochemical and biophysical research communications
Volume461
Issue number3
DOIs
Publication statusPublished - 2015 Jun 12

Keywords

  • Calpain
  • Cell cycle
  • MYCN
  • Myc-nick
  • NCYM
  • Neuroblastoma

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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