Nature of the Chemical Bond Formed with the Structural Metal Ion at the A9/G10.1 Motif Derived from Hammerhead Ribozymes

Yoshiyuki Tanaka, Yasuhiro Kasai, Shunsuke Mochizuki, Akihiro Wakisaka, Eugene H. Morita, Chojiro Kojima, Atsushi Toyozawa, Yoshinori Kondo, Masumi Taki, Yasuomi Takagi, Atsushi Inoue, Kazuhiko Yamasaki, Kazunari Taira

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

We have studied the interaction between metal ions and the metal ion-binding motif in hammerhead ribozymes, as well as the functions of the metal ion at the motif, with heteronuclear NMR spectroscopy. In this study, we employed model RNA systems which mimic the metal ion-binding motif and the altered motif. In Co(NH3)6(III) titrations, we observed large 1H and 31P chemical shift perturbations for the motif and found that outer-sphere complexation of Co(NH3) 6(III) is possible for this motif. From the reinvestigation of our previous 15N chemical shift data for Cd(II) binding, in comparison with those of organometallic compounds, we conclude that Cd(II) can form an inner-sphere complex with the nucleobase in the motif. Therefore, the A9/G10.1 site was found to accept both inner-sphere and outer-sphere complexations. The Mg(II) titration for a slightly different motif from the A9/G10.1 site (G10.1-C11.1 to A10.1-U11.1) revealed that its affinity to Mg(II) was drastically reduced, although the ribozyme with this altered motif is known to retain enzymatic activities. This observation suggests that the metal ion at these motifs is not a catalytic center of hammerhead ribozymes.

Original languageEnglish
Pages (from-to)744-752
Number of pages9
JournalJournal of the American Chemical Society
Volume126
Issue number3
DOIs
Publication statusPublished - 2004 Jan 28

ASJC Scopus subject areas

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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