Native sulfur/chlorine SAD phasing for serial femtosecond crystallography

Takanori Nakane, Changyong Song, Mamoru Suzuki, Eriko Nango, Jun Kobayashi, Tetsuya Masuda, Shigeyuki Inoue, Eiichi Mizohata, Toru Nakatsu, Tomoyuki Tanaka, Rie Tanaka, Tatsuro Shimamura, Kensuke Tono, Yasumasa Joti, Takashi Kameshima, Takaki Hatsui, Makina Yabashi, Osamu Nureki, So Iwata, Michihiro Sugahara

Research output: Contribution to journalArticlepeer-review

41 Citations (Scopus)

Abstract

Serial femtosecond crystallography (SFX) allows structures to be determined with minimal radiation damage. However, phasing native crystals in SFX is not very common. Here, the structure determination of native lysozyme from single-wavelength anomalous diffraction (SAD) by utilizing the anomalous signal of sulfur and chlorine at a wavelength of 1.77 Å is successfully demonstrated. This sulfur SAD method can be applied to a wide range of proteins, which will improve the determination of native crystal structures.

Original languageEnglish
Pages (from-to)2519-2525
Number of pages7
JournalActa Crystallographica Section D: Biological Crystallography
Volume71
DOIs
Publication statusPublished - 2015
Externally publishedYes

Keywords

  • Serial femtosecond crystallography
  • Sulfur/chlorine SAD
  • X-ray free-electron laser

ASJC Scopus subject areas

  • Structural Biology

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