Nasopharyngeal-associated lymphoreticular tissue (NALT) immunity: Fimbriae-specific Th1 and Th2 cell-regulated IgA responses for the inhibition of bacterial attachment to epithelial cells and subsequent inflammatory cytokine production

Manabu Yanagita, Takachika Hiroi, Noriko Kitagaki, Shigeyuki Hamada, Ito Hiro-o, Hidetoshi Shimauchi, Shinya Murakami, Hiroshi Okada, Hiroshi Kiyono

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)

Abstract

To investigate the antibacterial activity of mucosal Th1 and Th2 immune responses induced nasally and orally, mice were immunized with mucosal vaccine containing fimbrial protein of Porphyromonas gingivalis, a causative agent for a destructive chronic inflammation in the periodontium, and cholera toxin (CT) as mucosal adjuvant. Nasal vaccine containing low doses of fimbriae (10 μg) and CT (1 μg) induced Ag-specific Th1/Th2-type response in CD4+ T cells in mucosal effector tissues, including nasal passage and submandibular glands, which accounted for the generation of Ag-specific IgA- producing cells. In contrast, oral 'immunization required higher amounts of fimbriae and CT for the induction of Ag-specific IgA responses. Fimbriae- specific IgA mAbs generated from submandibular glands of nasally immunized mice inhibited P. gingivalis attachment to and reduced subsequent inflammatory cytokine production from epithelial cells. These findings suggest that nasal vaccination is an effective immunization regimen for the induction of Ag-specific Th1 and Th2 cell-driven IgA immune responses that possess the ability to inhibit bacterial attachment to epithelial cells and subsequent inflammatory cytokine production.

Original languageEnglish
Pages (from-to)3559-3565
Number of pages7
JournalJournal of Immunology
Volume162
Issue number6
Publication statusPublished - 1999 Mar 15

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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