Nardosinone enhances nerve growth factor-induced neurite outgrowth in a mitogen-activated protein kinase- and protein kinase C-dependent manner in PC12D cells

Ping Li, Tohru Yamakuni, Kimihiro Matsunaga, Shunzo Kondo, Yasushi Ohizumi

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

The mechanism to enhance nerve growth factor (NGF, 2 ng/ml)-induced neurite outgrowth from PC12D cells by nardosinone isolated from Nardostachys chinensis was examined. It was shown that the potentiation of the NGF-induced neurite outgrowth by nardosinone was mitogen-activated protein (MAP) kinase-dependent, but was not accompanied by stimulation of NGF-induced increase in MAP kinase phosphorylation. Furthermore, this augmentation of NGF-induced neurite outgrowth was abolished by GF109203X, a protein kinase C (PKC) inhibitor. These results suggest that the enhancement of NGF-induced neurite outgrowth from PC12D cells by nardosinone involves activation of a down-stream step of the MAP kinase-dependent cascade of NGF coupled with PKC.

Original languageEnglish
Pages (from-to)122-125
Number of pages4
JournalJournal of Pharmacological Sciences
Volume93
Issue number1
DOIs
Publication statusPublished - 2003 Sep 1

Keywords

  • Nardosinone
  • Nerve growth factor-induced neurite outgrowth
  • PC12D cell

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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