Nanostructured phospholipid biointerface for immunoassay microchip integrated with plasma separation membrane

Kazuki Nishizawa, Tomohiro Konno, Madoka Takai, Kazuhiko Ishihara

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

We developed both non-biofouling plasma separation membrane and solid phase of high sensitive immunoassay for the pre-treatment-free one-chip immunoassay device. The nanofiber for plasma separation membrane and nanosphere for the solid phase of the immunoassay were made by electrospray deposition (ESD) with 2-methacryloyloxyethyl phosphorylcholine (MPC) based polymer which shows a high resistivity to protein adsorption. The MPC polymer modified poly(vinyl alcohol) nanofiber membrane reduced the adsorption of the blood cells after immersing the whole blood. Immunoassay in the microchip using nanosphere shaped bioconjugatable MPC polymer was carried out and the chemiluminescence intensity corresponding to the concentration of analyte was observed.

Original languageEnglish
Title of host publicationProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
EditorsJean-Louis Viovy, Patrick Tabeling, Stephanie Descroix, Laurent Malaquin
PublisherChemical and Biological Microsystems Society
Pages1291-1293
Number of pages3
ISBN (Electronic)9780979806407
Publication statusPublished - 2007
Event11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 - Paris, France
Duration: 2007 Oct 72007 Oct 11

Publication series

NameProceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

Conference

Conference11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007
CountryFrance
CityParis
Period07/10/707/10/11

Keywords

  • Elctrospray
  • Immunoassay
  • MPC polymer
  • Nanofiber

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Nishizawa, K., Konno, T., Takai, M., & Ishihara, K. (2007). Nanostructured phospholipid biointerface for immunoassay microchip integrated with plasma separation membrane. In J-L. Viovy, P. Tabeling, S. Descroix, & L. Malaquin (Eds.), Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007 (pp. 1291-1293). (Proceedings of the 11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007). Chemical and Biological Microsystems Society.