Nanoscale imaging of an unlabeled secretory protein in living cells using scanning ion conductance microscopy

Yuji Nashimoto, Yasufumi Takahashi, Hiroki Ida, Yoshiharu Matsumae, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

Scanning ion conductance microscopy (SICM) was applied to evaluate an unlabeled secretory protein in living cells. The target protein, von Willebrand factor (vWF), was released from human endothelial cells by adding phorbol-12-myristate-13-acetate (PMA). We confirmed that SICM could be used to clearly visualize the complex network of vWF and to detect strings with widths as low as 60 nm without any artifact. By acquiring the sequential SICM images of living cells, the protrusion and strings formation were observed. We also detected the opening and closing motions of a small pore (∼500 nm), which is difficult to visualize with fluorescence methods. The results clearly demonstrate that SICM is a powerful tool to examine the changes in living cells during exocytosis.

Original languageEnglish
Pages (from-to)2542-2545
Number of pages4
JournalAnalytical Chemistry
Volume87
Issue number5
DOIs
Publication statusPublished - 2015 Mar 3

ASJC Scopus subject areas

  • Analytical Chemistry

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