TY - JOUR
T1 - Nanorheology measurement on single circularly permuted green fluorescent protein molecule
AU - Wang, Tong
AU - Sakai, Yasuhiro
AU - Nakajima, Ken
AU - Miyawaki, Atsushi
AU - Ito, Kohzo
AU - Hara, Masahiko
N1 - Funding Information:
This work was supported by a grant from Japan Society for the Promotion of Science (JSPS) to T.W. and by a President's Special Research Grant from RIKEN to K.N. and A.M.
PY - 2005/2/25
Y1 - 2005/2/25
N2 - The force measurement mode of an atomic force microscope (AFM) has enabled us to measure the mechanical properties of biological materials at the single molecular level. In a conventional quasi-static force measurement on a single circularly permuted green fluorescent protein (cpGFP), we could unfold it by unraveling several sub-domains in a distinct sawtooth pattern at a slow stretching speed. In order to elucidate more detailed conformational changes at each extension length, we further measured dynamic relax-stress response of cpGFP molecules. In this measurement, several cycles of sinusoidal movement were applied to the sample during the stretching process. We found the protein molecule showed in-phase response to the sinusoidal input in most case of measurements.
AB - The force measurement mode of an atomic force microscope (AFM) has enabled us to measure the mechanical properties of biological materials at the single molecular level. In a conventional quasi-static force measurement on a single circularly permuted green fluorescent protein (cpGFP), we could unfold it by unraveling several sub-domains in a distinct sawtooth pattern at a slow stretching speed. In order to elucidate more detailed conformational changes at each extension length, we further measured dynamic relax-stress response of cpGFP molecules. In this measurement, several cycles of sinusoidal movement were applied to the sample during the stretching process. We found the protein molecule showed in-phase response to the sinusoidal input in most case of measurements.
KW - Atomic force microscope
KW - Green fluorescent protein
KW - Mechanical unfolding
KW - Nanorheology measurement
KW - Sinusoidal modulation
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U2 - 10.1016/j.colsurfb.2004.10.012
DO - 10.1016/j.colsurfb.2004.10.012
M3 - Article
C2 - 15708511
AN - SCOPUS:13644269340
VL - 40
SP - 183
EP - 187
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
IS - 3-4 SPEC. ISS.
ER -