Nanorheology measurement on single circularly permuted green fluorescent protein molecule

Tong Wang, Yasuhiro Sakai, Ken Nakajima, Atsushi Miyawaki, Kohzo Ito, Masahiko Hara

    Research output: Contribution to journalArticlepeer-review

    11 Citations (Scopus)

    Abstract

    The force measurement mode of an atomic force microscope (AFM) has enabled us to measure the mechanical properties of biological materials at the single molecular level. In a conventional quasi-static force measurement on a single circularly permuted green fluorescent protein (cpGFP), we could unfold it by unraveling several sub-domains in a distinct sawtooth pattern at a slow stretching speed. In order to elucidate more detailed conformational changes at each extension length, we further measured dynamic relax-stress response of cpGFP molecules. In this measurement, several cycles of sinusoidal movement were applied to the sample during the stretching process. We found the protein molecule showed in-phase response to the sinusoidal input in most case of measurements.

    Original languageEnglish
    Pages (from-to)183-187
    Number of pages5
    JournalColloids and Surfaces B: Biointerfaces
    Volume40
    Issue number3-4 SPEC. ISS.
    DOIs
    Publication statusPublished - 2005 Feb 25

    Keywords

    • Atomic force microscope
    • Green fluorescent protein
    • Mechanical unfolding
    • Nanorheology measurement
    • Sinusoidal modulation

    ASJC Scopus subject areas

    • Biotechnology
    • Surfaces and Interfaces
    • Physical and Theoretical Chemistry
    • Colloid and Surface Chemistry

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