Nanoparticle Assisted Remodeling of Proteotoxic SOD1 Mutants Alters the Biointerface of the Functional Interaction of Microtubules and Kinesin Motors

Kyongwan Kim, Selvaraj Subramaniyam, Ahmad Galaleldeen, Hikaru Nakazawa, Mitsuo Umetsu, Winfried Teizer, Sanjib Bhattacharyya

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Transport deficits with motor neuron degeneration have been implicated in amyotrophic lateral sclerosis (ALS). We report a biomimetic system composed of microtubules/kinesin motor that mimics the dysregulated motor dynamics of ALS. Pathogenic ALS mutants A4V SOD1 completely shut off motility. Treatment with 5 nm citrate coated gold nanoparticles recovers the impaired motor stepping by remodeling the A4V SOD1 rather than stabilizing microtubules or protein folding. Instead, gold nanoparticles alter the protein by a mechanism that reforms protein elements of A4V SOD1, in turn fixing the aberrant interaction of kinesin with microtubules. Reinstating kinesin motility holds potential for managing debilitating ALS.

Original languageEnglish
Pages (from-to)4121-4128
Number of pages8
JournalACS Applied Bio Materials
Volume2
Issue number10
DOIs
Publication statusPublished - 2019 Oct 21

Keywords

  • Biomimicking transport
  • Proteotoxicity
  • gold nanopaticles
  • microtubule self-assembly
  • motor restorations
  • mutant superoxide dismutase 1 (SOD1)
  • quantum dot kinesin

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomaterials
  • Biomedical Engineering
  • Biochemistry, medical

Fingerprint

Dive into the research topics of 'Nanoparticle Assisted Remodeling of Proteotoxic SOD1 Mutants Alters the Biointerface of the Functional Interaction of Microtubules and Kinesin Motors'. Together they form a unique fingerprint.

Cite this