Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Yoshiyuki Manabe; Takanori Sugimoto; Tomoyuki Kawasaki; Minoru Ueda

doi:10.1016/j.tetlet.2006.12.128

Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Yoshiyuki Manabe, Takanori Sugimoto, Tomoyuki Kawasaki, Minoru Ueda

SCI - Chemistry

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

Original language	English
Pages (from-to)	1341-1344
Number of pages	4
Journal	Tetrahedron Letters
Volume	48
Issue number	8
DOIs	https://doi.org/10.1016/j.tetlet.2006.12.128
Publication status	Published - 2007 Feb 19

Keywords

Leaf-opening substance
Nano-gold
Nyctinasty
TEM
TEM probe

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

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title = "Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe",

abstract = "Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.",

keywords = "Leaf-opening substance, Nano-gold, Nyctinasty, TEM, TEM probe",

author = "Yoshiyuki Manabe and Takanori Sugimoto and Tomoyuki Kawasaki and Minoru Ueda",

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T1 - Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

AU - Manabe, Yoshiyuki

AU - Sugimoto, Takanori

AU - Kawasaki, Tomoyuki

AU - Ueda, Minoru

PY - 2007/2/19

Y1 - 2007/2/19

N2 - Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

AB - Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

KW - Leaf-opening substance

KW - Nano-gold

KW - Nyctinasty

KW - TEM

KW - TEM probe

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JO - Tetrahedron Letters

JF - Tetrahedron Letters

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