Abstract
Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.
Original language | English |
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Pages (from-to) | 1341-1344 |
Number of pages | 4 |
Journal | Tetrahedron Letters |
Volume | 48 |
Issue number | 8 |
DOIs | |
Publication status | Published - 2007 Feb 19 |
Keywords
- Leaf-opening substance
- Nano-gold
- Nyctinasty
- TEM
- TEM probe
ASJC Scopus subject areas
- Biochemistry
- Drug Discovery
- Organic Chemistry