Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Yoshiyuki Manabe; Takanori Sugimoto; Tomoyuki Kawasaki; Minoru Ueda

doi:10.1016/j.tetlet.2006.12.128

Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Yoshiyuki Manabe, Takanori Sugimoto, Tomoyuki Kawasaki, Minoru Ueda

SCI - Chemistry

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

Original language	English
Pages (from-to)	1341-1344
Number of pages	4
Journal	Tetrahedron Letters
Volume	48
Issue number	8
DOIs	https://doi.org/10.1016/j.tetlet.2006.12.128
Publication status	Published - 2007 Feb 19

Keywords

Leaf-opening substance
Nano-gold
Nyctinasty
TEM
TEM probe

ASJC Scopus subject areas

Biochemistry
Drug Discovery
Organic Chemistry

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10.1016/j.tetlet.2006.12.128

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@article{eddb15c49ae84c1eb662d43eb05bf49b,

title = "Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe",

abstract = "Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.",

keywords = "Leaf-opening substance, Nano-gold, Nyctinasty, TEM, TEM probe",

author = "Yoshiyuki Manabe and Takanori Sugimoto and Tomoyuki Kawasaki and Minoru Ueda",

note = "Funding Information: This work was supported by Grant-in-Aid for Scientific Research on Priority Area 16073216 from the Ministry of Education, Science, Sports, and Culture (MEXT), Grant-in-Aid for Scientific Research (No. 15681013). We also thank the Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku University, for the cultivation of Cassia plant. And we thank Professor Masayuki Inoue (Tohoku University) for useful discussion on the use of nano-gold particle.",

year = "2007",

month = feb,

day = "19",

doi = "10.1016/j.tetlet.2006.12.128",

language = "English",

volume = "48",

pages = "1341--1344",

journal = "Tetrahedron Letters",

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TY - JOUR

T1 - Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

AU - Manabe, Yoshiyuki

AU - Sugimoto, Takanori

AU - Kawasaki, Tomoyuki

AU - Ueda, Minoru

N1 - Funding Information: This work was supported by Grant-in-Aid for Scientific Research on Priority Area 16073216 from the Ministry of Education, Science, Sports, and Culture (MEXT), Grant-in-Aid for Scientific Research (No. 15681013). We also thank the Experimental Station for Medicinal Plant Studies, Graduate School of Pharmaceutical Sciences, Tohoku University, for the cultivation of Cassia plant. And we thank Professor Masayuki Inoue (Tohoku University) for useful discussion on the use of nano-gold particle.

PY - 2007/2/19

Y1 - 2007/2/19

N2 - Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

AB - Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

KW - Leaf-opening substance

KW - Nano-gold

KW - Nyctinasty

KW - TEM

KW - TEM probe

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DO - 10.1016/j.tetlet.2006.12.128

M3 - Article

AN - SCOPUS:33846356654

VL - 48

SP - 1341

EP - 1344

JO - Tetrahedron Letters

JF - Tetrahedron Letters

SN - 0040-4039

IS - 8

ER -

Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Abstract

Keywords

ASJC Scopus subject areas

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