Nanometer-scale direct observation of the receptor for the leaf-movement factor in plant cell by a novel TEM probe

Yoshiyuki Manabe, Takanori Sugimoto, Tomoyuki Kawasaki, Minoru Ueda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Electron microscopy is a useful method for observing localization of some proteins in a cell. In this Letter, we report a nanometer-scale direct observation of the receptor for a bioactive substance of small molecular weight by using TEM (transmission electron microscopy). We developed a novel TEM probe compound (1) that is a modified leaf-movement factor with benzophenone as a photoaffinity group and FITC as an antigen that is recognized by a nano-gold bound anti-FITC antibody. By using probe 1, we revealed that the receptor for the leaf-movement factor of Cassia mimosoides is localized in the plasma membrane of the plant motor cell.

Original languageEnglish
Pages (from-to)1341-1344
Number of pages4
JournalTetrahedron Letters
Volume48
Issue number8
DOIs
Publication statusPublished - 2007 Feb 19

Keywords

  • Leaf-opening substance
  • Nano-gold
  • Nyctinasty
  • TEM
  • TEM probe

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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