TY - JOUR
T1 - Nanohydroxyapatite increases BMP-2 expression via a p38 MAP kinase dependent pathway in periodontal ligament cells
AU - Suto, Mizuki
AU - Nemoto, Eiji
AU - Kanaya, Sousuke
AU - Suzuki, Risako
AU - Tsuchiya, Masahiro
AU - Shimauchi, Hidetoshi
N1 - Funding Information:
This work was supported by a Grant-in-Aid for Scientific Research (21390552 and 23390475) and Grant-in-Aid for Exploratory Research (21659437) from the Japan Society for the Promotion of Science.
PY - 2013/8
Y1 - 2013/8
N2 - Objective: Bone morphogenetic protein (BMP)-2 promotes the osteoblastic differentiation of human periodontal ligament (PDL) cells, which play a pivotal role in periodontal regeneration. Recently, nano-sized hydroxyapatite (nano-HA) has been highlighted due to its advantageous features over micro-sized materials. Design and results: We investigated the effect of nano-HA on BMP-2 expression in human PDL cells. Real time PCR analysis revealed that the expression of BMP-2 increased upon stimulation with nano-HA in dose- and time-dependent manners. An immunofluorescence assay demonstrated the synthesis of BMP-2 proteins. Concentrations of Ca2+ as well as phosphate (Pi) in culture supernatants were unchanged, suggesting that nano-HA functioned as a nanoparticle rather than as a possible source for releasing Ca2+ and/or Pi extracellularly, which were shown to also enhance the expression of BMP-2. Nano-HA-induced BMP-2 expression was dependent on the p38 MAP kinase pathway because increases in BMP-2 expression were inhibited by treatment with SB203580, a p38 inhibitor, and phosphorylation of p38 was detected by Western blotting. Conclusions: This novel mechanism of nano-HA will be important for the rational design of future periodontal regeneration.
AB - Objective: Bone morphogenetic protein (BMP)-2 promotes the osteoblastic differentiation of human periodontal ligament (PDL) cells, which play a pivotal role in periodontal regeneration. Recently, nano-sized hydroxyapatite (nano-HA) has been highlighted due to its advantageous features over micro-sized materials. Design and results: We investigated the effect of nano-HA on BMP-2 expression in human PDL cells. Real time PCR analysis revealed that the expression of BMP-2 increased upon stimulation with nano-HA in dose- and time-dependent manners. An immunofluorescence assay demonstrated the synthesis of BMP-2 proteins. Concentrations of Ca2+ as well as phosphate (Pi) in culture supernatants were unchanged, suggesting that nano-HA functioned as a nanoparticle rather than as a possible source for releasing Ca2+ and/or Pi extracellularly, which were shown to also enhance the expression of BMP-2. Nano-HA-induced BMP-2 expression was dependent on the p38 MAP kinase pathway because increases in BMP-2 expression were inhibited by treatment with SB203580, a p38 inhibitor, and phosphorylation of p38 was detected by Western blotting. Conclusions: This novel mechanism of nano-HA will be important for the rational design of future periodontal regeneration.
KW - BMP-2
KW - MAP kinase
KW - Nanohydroxyapatite
KW - Periodontal ligament cells
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U2 - 10.1016/j.archoralbio.2013.02.014
DO - 10.1016/j.archoralbio.2013.02.014
M3 - Article
C2 - 23518236
AN - SCOPUS:84879249702
VL - 58
SP - 1021
EP - 1028
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
IS - 8
ER -