Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Urara Hasegawa; Shin Ichiro M. Nomura; Sunil C. Kaul; Takashi Hirano; Kazunari Akiyoshi

doi:10.1016/j.bbrc.2005.03.228

Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Urara Hasegawa, Shin Ichiro M. Nomura, Sunil C. Kaul, Takashi Hirano, Kazunari Akiyoshi

Research output: Contribution to journal › Article › peer-review

185 Citations (Scopus)

Abstract

We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH₂). The CHPNH₂-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.

Original language	English
Pages (from-to)	917-921
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	331
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.228
Publication status	Published - 2005 Jun 17
Externally published	Yes

Keywords

Bioimaging
Carrier
Cationic liposome
Cellular uptake
Cholesterol-bearing pullulan
Live cell labeling
Nanogel
Quantum dot

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1016/j.bbrc.2005.03.228

Cite this

@article{e71931a07dab4575894fe50f98507cf7,

title = "Nanogel-quantum dot hybrid nanoparticles for live cell imaging",

abstract = "We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.",

keywords = "Bioimaging, Carrier, Cationic liposome, Cellular uptake, Cholesterol-bearing pullulan, Live cell labeling, Nanogel, Quantum dot",

author = "Urara Hasegawa and Nomura, {Shin Ichiro M.} and Kaul, {Sunil C.} and Takashi Hirano and Kazunari Akiyoshi",

year = "2005",

month = jun,

day = "17",

doi = "10.1016/j.bbrc.2005.03.228",

language = "English",

volume = "331",

pages = "917--921",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

publisher = "Academic Press Inc.",

number = "4",

}

TY - JOUR

T1 - Nanogel-quantum dot hybrid nanoparticles for live cell imaging

AU - Hasegawa, Urara

AU - Nomura, Shin Ichiro M.

AU - Kaul, Sunil C.

AU - Hirano, Takashi

AU - Akiyoshi, Kazunari

PY - 2005/6/17

Y1 - 2005/6/17

N2 - We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.

AB - We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.

KW - Bioimaging

KW - Carrier

KW - Cationic liposome

KW - Cellular uptake

KW - Cholesterol-bearing pullulan

KW - Live cell labeling

KW - Nanogel

KW - Quantum dot

UR - http://www.scopus.com/inward/record.url?scp=18844425201&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18844425201&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.03.228

DO - 10.1016/j.bbrc.2005.03.228

M3 - Article

C2 - 15882965

AN - SCOPUS:18844425201

VL - 331

SP - 917

EP - 921

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 4

ER -

Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this