Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Urara Hasegawa; Shin Ichiro M. Nomura; Sunil C. Kaul; Takashi Hirano; Kazunari Akiyoshi

doi:10.1016/j.bbrc.2005.03.228

Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Urara Hasegawa, Shin Ichiro M. Nomura, Sunil C. Kaul, Takashi Hirano, Kazunari Akiyoshi

Research output: Contribution to journal › Article › peer-review

170 Citations (Scopus)

Abstract

We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH₂). The CHPNH₂-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.

Original language	English
Pages (from-to)	917-921
Number of pages	5
Journal	Biochemical and biophysical research communications
Volume	331
Issue number	4
DOIs	https://doi.org/10.1016/j.bbrc.2005.03.228
Publication status	Published - 2005 Jun 17
Externally published	Yes

Keywords

Bioimaging
Carrier
Cationic liposome
Cellular uptake
Cholesterol-bearing pullulan
Live cell labeling
Nanogel
Quantum dot

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2005.03.228

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title = "Nanogel-quantum dot hybrid nanoparticles for live cell imaging",

abstract = "We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.",

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AU - Hasegawa, Urara

AU - Nomura, Shin Ichiro M.

AU - Kaul, Sunil C.

AU - Hirano, Takashi

AU - Akiyoshi, Kazunari

PY - 2005/6/17

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AB - We report here a novel carrier of quantum dots (QDs) for intracellular labeling. Monodisperse hybrid nanoparticles (38 nm in diameter) of QDs were prepared by simple mixing with nanogels of cholesterol-bearing pullulan (CHP) modified with amino groups (CHPNH2). The CHPNH2-QD nanoparticles were effectively internalized into the various human cells examined. The efficiency of cellular uptake was much higher than that of a conventional carrier, cationic liposome. These hybrid nanoparticles could be a promising fluorescent probe for bioimaging.

KW - Bioimaging

KW - Carrier

KW - Cationic liposome

KW - Cellular uptake

KW - Cholesterol-bearing pullulan

KW - Live cell labeling

KW - Nanogel

KW - Quantum dot

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Nanogel-quantum dot hybrid nanoparticles for live cell imaging

Abstract

Keywords

ASJC Scopus subject areas

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