Nano-Resolution X-ray tomography for deciphering wiring diagram of mammalianbrain

H. Mizutani, H. Sagara, A. Takeuchi, T. Ohigashi, W. Yashiro, K. Uesugi, Y. Suzuki, A. Momose, T. Takagi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Neural circuits in the central nervous system are the substrate of various high-order brain functions. However, little is known about the mechanisms underlying neuronal information processing in the brain. Anatomical and functional graph structures of neural networks with actual connections will provide us with perspectives to elucidate the brain complexity. Here, we aim to develop a three-dimensional mouse brain atlas of neural circuits using nano-resolution x-ray tomography by synchrotron radiation. In addition to identifying a large number of synapses, our research will also clarify the structure of neuronal networks for understanding the most complex organ in the body. In this study, we observed metal-stained biological tissues of the mouse brain using hard x-ray Zernike-type phase-contrast microscopy with 60-nm resolution at SPring-8. As a result, the nano-resolution hard x-ray phase-contrast microscope revealed nerve fibers and organelles including mitochondria and synapses in the neural tissue. In the near future, this information will be utilized to begin deciphering the wiring diagram of the brain by using nano-resolution x-ray tomography.

Original languageEnglish
Title of host publication10th International Conference on X-Ray Microscopy
Pages399-402
Number of pages4
DOIs
Publication statusPublished - 2010 Dec 1
Externally publishedYes
Event10th International Conference on X-Ray Microscopy - Chicago, IL, United States
Duration: 2010 Aug 152010 Aug 20

Publication series

NameAIP Conference Proceedings
Volume1365
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

Other10th International Conference on X-Ray Microscopy
Country/TerritoryUnited States
CityChicago, IL
Period10/8/1510/8/20

Keywords

  • Synaptic connections
  • brain
  • mouse
  • neuronal networks
  • x-ray phase contrast imaging

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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