NAADP triggers the fertilization potential in starfish oocytes

Francesco Moccia, Dmitry Lim, Keiichiro Kyozuka, Luigia Santella

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

In invertebrates oocytes or eggs, the fertilization or activation potential establishes the fast electrical block to polyspermy and, in some species, provides the Ca2+ influx which contributes to the following intracellular Ca2+ wave. In echinoderms, the molecule triggering the activation potential is still unknown. The aim of this study was to assess whether nicotinic acid-adenine dinucleotide phosphate (NAADP) elicited the fertilization potential in starfish oocytes. The changes in membrane potential induced by the sperm were measured in oocytes held at a low resting potential, so that the Ca2+-action potential was inactivated and only the initial slower depolarization caused by the sperm could be studied. Decreasing extracellular Na+ concentration did not prevent the onset of the fertilization potential, while removal of external Ca2+ abolished it. The pre-incubation with SK&F 96365 and verapamil and the pre-injection of BAPTA inhibited the fertilization potential, while the injection of heparin only reduced its duration. The biophysical and pharmacological properties of the sperm-elicited depolarization were similar to those displayed by the NAADP-activated Ca2+-mediated current recently described in starfish oocytes. Indeed, the desensitization of NAADP-receptors prevented the onset of the fertilization potential. Taken together, these data suggest that NAADP could trigger the fertilization potential in starfish oocytes.

Original languageEnglish
Pages (from-to)515-524
Number of pages10
JournalCell Calcium
Volume36
Issue number6
DOIs
Publication statusPublished - 2004 Dec

Keywords

  • Calcium
  • Fertilization
  • NAADP
  • Oocyte
  • Starfish

ASJC Scopus subject areas

  • Physiology
  • Molecular Biology
  • Cell Biology

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