Abstract
[N-Methyl-11C]choline has been synthesized at room temperature by the reaction of [11C]CH3I with 2-dimethylaminoethanol (DMAE), with the latter directly loaded on a weak cation-exchange cartridge. Most of the efforts have been directed to reduce the amount of residual precursor in the product's final solution in order to make this tracer more suitable to brain studies. In the process, radiochemical yields and residual DMAE have been placed in relation with both the starting amount of precursor and the rinsing conditions used and compared with the more 'traditional' loading of the precursor on either a C18 cartridge or a loop. Comments and indications on the most convenient analytical technique and conditions for quantitative analysis, with particular emphasis on the precursor, are also reported. Under what we believe to be a fair compromise, [11C]CH3I incorporation yields of ca. 90% were easily achieved with a residual amount of starting material in the 8- to 12-ppm range.
Original language | English |
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Pages (from-to) | 157-162 |
Number of pages | 6 |
Journal | Journal of Labelled Compounds and Radiopharmaceuticals |
Volume | 54 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2011 Mar |
Externally published | Yes |
Keywords
- 2-dimethylamino ethanol
- choline
- on-column
- quality control
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Radiology Nuclear Medicine and imaging
- Drug Discovery
- Spectroscopy
- Organic Chemistry