N-acetylglucosaminyltransferase V and β1-6 branching N-linked oligosaccharides are associated with good prognosis of patients with bladder cancer

Hirofumi Ishimura, Toshiko Takahashi, Hiroaki Nakagawa, Shin Ichiro Nishimura, Yoichi Arai, Yohei Horikawa, Tomonori Habuchi, Eiji Miyoshi, Atsushi Kyan, Shigeru Hagisawa, Chikara Ohyama

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

Purpose: N-acetylglucosaminyltransferaseV (GnT-V) is an enzyme that catalyzes β1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides (N-glycan) of cell proteins. We examined the relationship between GnT-V expression and clinicopathologic features of the patients with bladder cancer. Experimental Design: We immunohistochemically examined GnT-V expression in paraffin-embedded bladder cancer specimen using anti-GnT-V monoclonal antibody. We compared GnT-V expression with cause-specific survival of the patients with bladder cancer treated by radical cystectomy. Kaplan-Meier survival curves were generated to show the cause-specific survival. Univariate and multivariate analyses were carried out to compare GnT-V expression with other clinical and pathologic variables. We also evaluated mRNA expression of GnT-V and N-linked oligosaccharide structure in bladder cancer specimens. Results: Immunohistochemistry revealed that GnT-V expression inversely correlated with tumor grade and stage. The incidence of positive GnT-V expression in bladder cancer was significantly higher in low-grade/superficial cancer than in high-grade/invasive cancer. The patients whose tumor was positive for GnT-V survived significantly longer than those whose tumor was negative for GnT-V. Univariate and multivariate analyses revealed that GnT-V expression was an independent predictor of prognosis of the patient. The expression of GnT-V mRNA determined by reverse transcription-PCR was consistent with the results with immunohistochemistry for tumor samples. Carbohydrate structural analysis revealed that superficial bladder cancer is rich in branched N-linked oligosaccharides, for which biosynthesis GnT-V is responsible. Conclusions: GnT-V and its resultant β1-6 branching N-linked oligosaccharides are closely related to low malignant potential and good prognosis of the patients with bladder cancer.

Original languageEnglish
Pages (from-to)2506-2511
Number of pages6
JournalClinical Cancer Research
Volume12
Issue number8
DOIs
Publication statusPublished - 2006 Apr 15

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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