Myogenic maturation by optical-training in cultured skeletal muscle cells

Toshifumi Asano, Toru Ishizuka, Hiromu Yawo

    Research output: Chapter in Book/Report/Conference proceedingChapter


    Optogenetic techniques are powerful tools for manipulating biological processes in identified cells using light under high temporal and spatial resolutions. Here, we describe an optogenetic training strategy to promote morphological maturation and functional development of skeletal muscle cells in vitro. Optical stimulation with a rhythmical frequency facilitates specific structural alignment of sarcomeric proteins. Optical stimulation also depolarizes the membrane potential, and induces contractile responses in synchrony with the given pattern of light pulses. These results suggest that optogenetic techniques can be employed to manipulate activity-dependent processes during myogenic development and control contraction of photosensitive skeletal muscle cells with high temporal and special precision.

    Original languageEnglish
    Title of host publicationMethods in Molecular Biology
    PublisherHumana Press Inc.
    Number of pages11
    Publication statusPublished - 2017

    Publication series

    NameMethods in Molecular Biology
    ISSN (Print)1064-3745


    • C2C12
    • Channelrhodopsin
    • Muscle contraction
    • Myogenesis
    • Optical stimulation
    • Optogenetics

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics


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