Mutations of the p53 Gene and Other Genes Involving in Human Colorectal Carcinogenesis

Ryunosuke Kanamaru, Chikashi Ishioka

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In this study, structural changes of the p53 gene in primary specimens of human colorectal carcinomas were analyzed by polymerase chain reaction mediated-DNA sequencing method. Point mutations of p53 gene, including an intronic mutation case, were detected in 8 of 14 carcinomas (57%). Point mutations of the gene were also observed in 2 of 2 adenomas, suggesting that mutations occur prior to the carcinoma stage. These results support that p53 gene plays an important role in the development of colorectal cancer. The frequency of Ki-ras oncogene mutations was also studied by polymerase chain reaction-single strand conformation polymorphism analysis (PCR-SSCP). This resulted in the rate of 42% (10/24), a quite similar value obtained by other methods. As PCR-SSCP analysis is a convenient method to detect point mutation, we have now examined 24 colorectal cancers for the p53 gene by this method, and detected the mutations. Furthermore, expression of the DCC gene, a candidate of tumor suppressor gene involved in colorectal carcinogenesis, was examined by reverse transcriptase-mediated PCR (RT-PCR) assay, resulting in significant reduction on the DCC expression in 8 of 14 carcinoma cases (57%).

Original languageEnglish
Pages (from-to)159-166
Number of pages8
JournalTohoku Journal of Experimental Medicine
Volume168
Issue number2
DOIs
Publication statusPublished - 1992

Keywords

  • DCC gene
  • Ki-ras gene
  • colorectal cancer
  • p53 gene

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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