Mutational effects of γ-Rays and carbon ion beams on arabidopsis seedlings

Ryouhei Yoshihara, Shigeki Nozawa, Yoshihiro Hase, Issay Narumi, Jun Hidema, Ayako N. Sakamoto

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    To assess the mutational effects of radiation on vigorously proliferating plant tissue, the mutation spectrum was analyzed with Arabidopsis seedlings using the plasmid-rescue method. Transgenic plants containing the Escherichia coli rpsL gene were irradiated with γ-rays and carbon ion beams (320-MeV 12C6+), and mutations in the rpsL gene were analyzed. Mutant frequency increased significantly following irradiation by γ-rays, but not by 320-MeV 12C6+. Mutation spectra showed that both radiations increased the frequency of frameshifts and other mutations, including deletions and insertions, but only γ-rays increased the frequency of total base substitutions. These results suggest that the type of DNA lesions which cause base substitutions were less often induced by 320-MeV 12C6+ than by γ-rays in Arabidopsis seedlings. Furthermore, γ-rays never increased the frequencies of G:C to T:A or A:T to C:G transversions, which are caused by oxidized guanine; 320-MeV 12C6+, however, produced a slight increase in both transversions. Instead, γ-rays produced a significant increase in the frequency of G:C to A:T transitions. These results suggest that 8-oxoguanine has little effect on mutagenesis in Arabidopsis cells.

    Original languageEnglish
    Pages (from-to)1050-1056
    Number of pages7
    JournalJournal of radiation research
    Volume54
    Issue number6
    DOIs
    Publication statusPublished - 2013 Nov

    Keywords

    • Arabidopsis
    • Carbon ion beams
    • Mutation spectrum
    • RpsL gene
    • γ-rays

    ASJC Scopus subject areas

    • Radiation
    • Radiology Nuclear Medicine and imaging
    • Health, Toxicology and Mutagenesis

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